Document Detail

Characterization of volatile metabolites taken up or released from Streptococcus pneumoniae and Haemophilus influenzae by gas chromatography mass spectrometry.
MedLine Citation:
PMID:  23059976     Owner:  NLM     Status:  Publisher    
Volatile organic compounds (VOCs) released or taken up by Streptococcus pneumoniae and Haemophilus influenzae cultures were analyzed by means of gas chromatography mass spectrometry (GC-MS) after adsorption of headspace samples on multibed sorption tubes. Sampling was performed at different time points during cultivation of bacteria to follow the dynamics of VOC metabolism. VOCs were identified not only by spectral library match, but also based on retention times of native standards.. As many as 34 volatile metabolites were released from Streptococcus pneumoniae and 28 from Haemophilus influenzae, comprising alcohols, aldehydes, esters, hydrocarbons, ketones and sulphur containing compounds. For both species, acetic acid, acetaldehyde, methyl methacrylate, 2,3-butanedione and methanethiol were found in strongly, 1-butanol and butanal in moderately elevated concentrations. In addition, characteristic volatile biomarkers were detected for both bacterial species and exclusively for S. pneumoniae also catabolism of aldehydes (3-methylbutanal and hexanal) was found. The results obtained provide important input into the knowledge about volatile bacterial biomarkers, which may become particularly important for detection of pathogens in upper airways by breath-gas analysis in the future.
Wojciech Filipiak; Andreas Sponring; Maria M Baur; Clemens Ager; Anna Filipiak; Helmut Wiesenhofer; Markus Nagl; Jakob Troppmair; Anton Amann
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-11
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  -     ISSN:  1465-2080     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Innsbruck Medical University.
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