Document Detail

Characterization of vitamin A-storing cells in mouse fibrous kidneys using Cygb/STAP as a marker of activated stellate cells.
MedLine Citation:
PMID:  17827667     Owner:  NLM     Status:  MEDLINE    
The expression of the cytoglobin/stellate cell activation-associated protein (Cygb/STAP) was recently confirmed in all splanchnic vitamin A-storing cells--including hepatic stellate cells (HSCs)--in normal conditions. In the hepatic fibrous lesion, the expression of Cygb/STAP has been shown to be upregulated in activated HSCs and myofibroblasts (MFs), which have synthesized extracellular matrices. Furthermore, splanchnic vitamin A-storing cells have been reported to be distributed in the kidney. In this study, we clarify the contribution of vitamin A-storing cells to renal fibrosis by focusing on Cygb/ STAP. Adult mice were subjected to unilateral ureteral obstruction (UUO) and kidneys were harvested 1, 3, 7, and 10 days after UUO. Numbers of Cygb/STAP-immunopositive cells as well as Cygb/STAP mRNA 3 days after UUO (UUO day 3 kidney) increased. Vitamin A-autofluorescence was observed in intertubular spaces of controls but gradually declined in a time-dependent manner after UUO. Cygb/STAP+ cells were not completely identical with alpha-smooth muscle actin (alphaSMA)-positive cells in the control or UUO day 7 kidneys. Immunohistochemical analysis for Cygb/STAP and fibulin-2 (Fib), a specific marker for distinguishing MFs from activated HSCs, revealed that the number of Fib+STAP+ cells (MFs) and Fib-STAP+ cells (splanchnic vitamin A-storing cells) significantly increased in UUO day 3 and UUO day 7 kidneys compared with the controls. Our present findings support the concept that Cygb/STAP can be a unique marker for splanchnic fibroblast-like cells, namely the vitamin A-storing cell lineage, and suggest that splanchnic vitamin A-storing cells contribute to renal fibrogenesis in the obstructed kidney.
Yujiro Kida; Kinji Asahina; Kouji Inoue; Norifumi Kawada; Katsutoshi Yoshizato; Kenjiro Wake; Tetsuji Sato
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of histology and cytology     Volume:  70     ISSN:  0914-9465     ISO Abbreviation:  Arch. Histol. Cytol.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-09-10     Completed Date:  2007-12-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806082     Medline TA:  Arch Histol Cytol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  95-106     Citation Subset:  IM    
Department of Anatomy II, School of Dental Medicine, Tsurumi University, Yokohama, Japan.
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MeSH Terms
Biological Markers / analysis,  metabolism
Fibrosis / metabolism*,  pathology
Fluorescent Antibody Technique
Gene Expression Regulation
Globins / analysis*,  genetics,  metabolism
Hepatocytes / chemistry*,  metabolism
Kidney / injuries,  metabolism*,  pathology
Kidney Diseases / metabolism*,  pathology
Mice, Inbred C57BL
Peroxidases / analysis*,  genetics,  metabolism
RNA, Messenger / biosynthesis
Splanchnic Nerves / cytology
Vitamin A / metabolism*,  pharmacology
Reg. No./Substance:
0/Biological Markers; 0/RNA, Messenger; 0/cytoglobin; 11103-57-4/Vitamin A; 9004-22-2/Globins; EC 1.11.1-/Staap protein, rat; EC 1.11.1.-/Peroxidases

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