| Characterization of vitamin A-storing cells in mouse fibrous kidneys using Cygb/STAP as a marker of activated stellate cells. | |
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MedLine Citation:
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PMID: 17827667 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The expression of the cytoglobin/stellate cell activation-associated protein (Cygb/STAP) was recently confirmed in all splanchnic vitamin A-storing cells--including hepatic stellate cells (HSCs)--in normal conditions. In the hepatic fibrous lesion, the expression of Cygb/STAP has been shown to be upregulated in activated HSCs and myofibroblasts (MFs), which have synthesized extracellular matrices. Furthermore, splanchnic vitamin A-storing cells have been reported to be distributed in the kidney. In this study, we clarify the contribution of vitamin A-storing cells to renal fibrosis by focusing on Cygb/ STAP. Adult mice were subjected to unilateral ureteral obstruction (UUO) and kidneys were harvested 1, 3, 7, and 10 days after UUO. Numbers of Cygb/STAP-immunopositive cells as well as Cygb/STAP mRNA 3 days after UUO (UUO day 3 kidney) increased. Vitamin A-autofluorescence was observed in intertubular spaces of controls but gradually declined in a time-dependent manner after UUO. Cygb/STAP+ cells were not completely identical with alpha-smooth muscle actin (alphaSMA)-positive cells in the control or UUO day 7 kidneys. Immunohistochemical analysis for Cygb/STAP and fibulin-2 (Fib), a specific marker for distinguishing MFs from activated HSCs, revealed that the number of Fib+STAP+ cells (MFs) and Fib-STAP+ cells (splanchnic vitamin A-storing cells) significantly increased in UUO day 3 and UUO day 7 kidneys compared with the controls. Our present findings support the concept that Cygb/STAP can be a unique marker for splanchnic fibroblast-like cells, namely the vitamin A-storing cell lineage, and suggest that splanchnic vitamin A-storing cells contribute to renal fibrogenesis in the obstructed kidney. |
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Authors:
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Yujiro Kida; Kinji Asahina; Kouji Inoue; Norifumi Kawada; Katsutoshi Yoshizato; Kenjiro Wake; Tetsuji Sato |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archives of histology and cytology Volume: 70 ISSN: 0914-9465 ISO Abbreviation: Arch. Histol. Cytol. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-09-10 Completed Date: 2007-12-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8806082 Medline TA: Arch Histol Cytol Country: Japan |
Other Details:
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Languages: eng Pagination: 95-106 Citation Subset: IM |
Affiliation:
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Department of Anatomy II, School of Dental Medicine, Tsurumi University, Yokohama, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers / analysis, metabolism Fibrosis / metabolism*, pathology Fluorescent Antibody Technique Gene Expression Regulation Globins / analysis*, genetics, metabolism Hepatocytes / chemistry*, metabolism Kidney / injuries, metabolism*, pathology Kidney Diseases / metabolism*, pathology Male Mice Mice, Inbred C57BL Peroxidases / analysis*, genetics, metabolism RNA, Messenger / biosynthesis Splanchnic Nerves / cytology Up-Regulation Vitamin A / metabolism*, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/RNA, Messenger; 0/cytoglobin; 11103-57-4/Vitamin A; 9004-22-2/Globins; EC 1.11.1-/Staap protein, rat; EC 1.11.1.-/Peroxidases |
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