Document Detail

Characterization of a vitamin B12 compound from unicellular coccolithophorid alga (Pleurochrysis carterae).
MedLine Citation:
PMID:  11453796     Owner:  NLM     Status:  MEDLINE    
A unicellular coccolithophorid alga, Pleurochrysis carterae, contained 125.4 +/- 1.2 microg of vitamin B12 per 100 g dry cell weight of the lyophilized algal cells. A vitamin B12 compound was purified from the lyophilized algal cells and partially characterized. The silica gel 60 TLC and reversed-phase HPLC patterns of the purified pink-colored compound were identical to those of authentic vitamin B12, but not those of vitamin B12 analogues inactive for humans. When 22-week-old B12-deficient rats which excreted substantial amounts of methylmalonic acid (75.5 +/- 12.3 mg/day) in urine were fed the P. carterae (10 g per kg diet)-supplemented diet for 12 d, urinary methylmalonic acid excretion (as an index of vitamin B12 deficiency) of the rats became undetectable and hepatic vitamin B12 level of the rats was significantly increased.
E Miyamoto; F Watanabe; S Ebara; S Takenaka; H Takenaka; Y Yamaguchi; N Tanaka; H Inui; Y Nakano
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  49     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-07-16     Completed Date:  2001-10-25     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3486-9     Citation Subset:  IM    
Department of Health Science, Kochi Women's University, Kochi 780-8515, Japan.
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MeSH Terms
Algae / metabolism*
Cells, Cultured
Chromatography, High Pressure Liquid
Methylmalonic Acid / urine
Time Factors
Vitamin B 12 / analogs & derivatives,  analysis,  isolation & purification*,  therapeutic use
Vitamin B 12 Deficiency / drug therapy*
Reg. No./Substance:
516-05-2/Methylmalonic Acid; 68-19-9/Vitamin B 12

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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