Document Detail

Characterization and virulence retention of viable but nonculturable Vibrio harveyi.
MedLine Citation:
PMID:  18248440     Owner:  NLM     Status:  MEDLINE    
Vibrio harveyi has been reported to enter into a viable but nonculturable (VBNC) state. Two clinical V. harveyi strains, SF1 and CW2, and the type strain, VIB295(T), were incubated in sterilized seawater at 4 degrees C. Plate counts in these strains declined to undetectable levels (<0.1 CFU mL(-1)) within 69, 67 and 65 days, respectively. The direct viable count (DVC) declined from 10(6) to 10(4) active cells mL(-1) and remained constant at this level by a DVC. VBNC cell numbers could be restored via a temperature upshift when grown in yeast extract with the addition of Tween 20 or compound vitamin B. Reverse transcriptase-PCR was used to monitor virulence gene expression within VBNC cells. No expression of the hemolysin gene was detected in VBNC cells. VBNC and resuscitative cells were intraperitoneally injected into zebra fish separately. No death was observed in the groups inoculated with VBNC cells. The fish inoculated with the resuscitative cells died within 7 days, the lethal dose 50% (LD(50)) being 2.85 x 10(4) CFU mL(-1), a value similar to that for groups inoculated with normal cells (2.28 x 10(4) CFU mL(-1)). This suggested that VBNC V. harveyi might retain pathogenic potential.
Fengrong Sun; Jixiang Chen; Linhong Zhong; Xiao-hua Zhang; Rong Wang; Qianru Guo; Yi Dong
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-31
Journal Detail:
Title:  FEMS microbiology ecology     Volume:  64     ISSN:  0168-6496     ISO Abbreviation:  FEMS Microbiol. Ecol.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-12     Completed Date:  2008-06-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8901229     Medline TA:  FEMS Microbiol Ecol     Country:  England    
Other Details:
Languages:  eng     Pagination:  37-44     Citation Subset:  IM    
Department of Marine Biology, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
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MeSH Terms
Bacterial Proteins / genetics,  metabolism
Colony Count, Microbial
Culture Media
Microbial Viability*
Seawater / microbiology*
Vibrio / classification,  growth & development*,  pathogenicity*,  physiology
Vibrio Infections / microbiology
Zebrafish / microbiology*
Reg. No./Substance:
0/Bacterial Proteins; 0/Culture Media

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