| Characterization of vesicular stomatitis virus recombinants that express and incorporate high levels of hepatitis C virus glycoproteins. | |
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MedLine Citation:
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PMID: 12072487 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We generated recombinant vesicular stomatitis viruses (VSV) expressing genes encoding hybrid proteins consisting of the extracellular domains of hepatitis C virus (HCV) glycoproteins fused at different positions to the transmembrane and cytoplasmic domains of the VSV G glycoprotein (E1G and E2G). We show that these chimeric proteins are transported to the cell surface and incorporated into VSV virions efficiently. We also generated VSV recombinants in which the gene encoding the VSV G protein was deleted and replaced by one or both of the E1G and E2G genes, together with a green fluorescent protein gene. These DeltaG viruses incorporated E1G and E2G proteins at levels approximately equivalent to the normal level of VSV G itself, or about 1,200 molecules of each protein per virion. Given the potency of VSV recombinants as vaccines in other studies, this high-level expression and incorporation of HCV proteins into virions could be very important for development of an HCV vaccine. Despite the presence of E1G and E2G proteins at high levels in the virions, these virions did not infect cell lines that have been reported to support at least a low level of HCV infection and replication. |
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Authors:
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Linda Buonocore; Keril J Blight; Charles M Rice; John K Rose |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of virology Volume: 76 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 2002 Jul |
Date Detail:
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Created Date: 2002-06-19 Completed Date: 2002-07-25 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: United States |
Other Details:
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Languages: eng Pagination: 6865-72 Citation Subset: IM |
Affiliation:
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Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cell Membrane / metabolism Hepacivirus* / genetics, metabolism, pathogenicity Humans Recombinant Fusion Proteins / genetics, metabolism Recombination, Genetic* Vesicular stomatitis Indiana virus / genetics*, pathogenicity Viral Envelope Proteins / genetics, metabolism* Virion / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AI24345/AI/NIAID NIH HHS; AI40034/AI/NIAID NIH HHS; CA57973/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/E1 protein, Hepatitis C virus; 0/Recombinant Fusion Proteins; 0/Viral Envelope Proteins; 157184-61-7/glycoprotein E2, Hepatitis C virus |
| Comments/Corrections | |
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