| Characterization of various blood and graft sources: a prospective series. | |
| | |
MedLine Citation:
|
PMID: 23320942 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
BACKGROUND: Studies comparing cell components of blood and graft sources are very scarce. We present here a thorough study examining the cellular content of various sources of blood and cell therapy products. STUDY DESIGN AND METHODS: We have prospectively compared by fluorescence-activated cell sorting analyses the cellular composition of three blood sources on the one hand-peripheral blood (PB; n = 10) versus granulocyte-colony-stimulating factor (G-CSF)-mobilized PB (GCSF-PB, n = 10) versus cord blood (CB, n = 10)-and of three graft sources on the other hand-unmanipulated bone marrow (uBM, n = 5) versus leukapheresis product (LP, n = 10) versus thawed CB graft (n = 7). RESULTS: All median absolute numbers of cell subsets were found significantly higher in GCSF-PB and LP, except for monocytoid dendritic cells (mDCs) in CB and uBM. The most impressive results were the median quantities of memory T and B lymphocytes but also of plasmacytoid DCs (pDCs) contained in LP compared to thawed CB graft, with ratios of 375, 318, and 247, respectively. The proportions of naive and CD4+/CD8- T cells, transitional B cells, and CD5+ and naive B lymphocytes were found significantly higher in CB samples while the proportions of mDCs and pDCs were found significantly lower. CONCLUSION: Our study shows strong differences in terms of quantitative and qualitative cellular composition between several blood or graft sources, possibly explaining the differences observed in terms of outcomes after transplant. |
| | |
Authors:
|
Patrice Chevallier; Nelly Robillard; Marina Illiaquer; Julie Esbelin; Mohamad Mohty; Celine Bodin-Bressollette; Thierry Guillaume; Veronique Stocco; Fabienne Auffray; Sophie Derenne; Lucie Planche; Marie-Christine Bene; Berthe-Marie Imbert-Marcille |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2013-1-16 |
Journal Detail:
|
Title: Transfusion Volume: - ISSN: 1537-2995 ISO Abbreviation: Transfusion Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-1-16 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0417360 Medline TA: Transfusion Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
© 2013 American Association of Blood Banks. |
Affiliation:
|
Service d'Hématologie Clinique, CHU Hôtel Dieu, Université de Nantes, Centre d'Investigation Clinique en Cancerologie (CI2C) and INSERM U892; Service d'Hematologie/Biologie, Service de Gynecologie/Obstetrique; Cellule de Promotion à la Recherche Clinique, CHU de Nantes; Service de Virologie, CHU de Nantes, and UPRES-EA4271, PRES L'UNAM, Nantes University; Unité d'Ingénierie Cellulaire, EFS Pays de la Loire, Nantes, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Vibrios adhere to epithelial cells in the intestinal tract of red sea bream, Pagrus major, utilizing...
Next Document: Fatherhood and cancer: a commentary on the literature.