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Characterization of various blood and graft sources: a prospective series.
MedLine Citation:
PMID:  23320942     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: Studies comparing cell components of blood and graft sources are very scarce. We present here a thorough study examining the cellular content of various sources of blood and cell therapy products. STUDY DESIGN AND METHODS: We have prospectively compared by fluorescence-activated cell sorting analyses the cellular composition of three blood sources on the one hand-peripheral blood (PB; n = 10) versus granulocyte-colony-stimulating factor (G-CSF)-mobilized PB (GCSF-PB, n = 10) versus cord blood (CB, n = 10)-and of three graft sources on the other hand-unmanipulated bone marrow (uBM, n = 5) versus leukapheresis product (LP, n = 10) versus thawed CB graft (n = 7). RESULTS: All median absolute numbers of cell subsets were found significantly higher in GCSF-PB and LP, except for monocytoid dendritic cells (mDCs) in CB and uBM. The most impressive results were the median quantities of memory T and B lymphocytes but also of plasmacytoid DCs (pDCs) contained in LP compared to thawed CB graft, with ratios of 375, 318, and 247, respectively. The proportions of naive and CD4+/CD8- T cells, transitional B cells, and CD5+ and naive B lymphocytes were found significantly higher in CB samples while the proportions of mDCs and pDCs were found significantly lower. CONCLUSION: Our study shows strong differences in terms of quantitative and qualitative cellular composition between several blood or graft sources, possibly explaining the differences observed in terms of outcomes after transplant.
Authors:
Patrice Chevallier; Nelly Robillard; Marina Illiaquer; Julie Esbelin; Mohamad Mohty; Celine Bodin-Bressollette; Thierry Guillaume; Veronique Stocco; Fabienne Auffray; Sophie Derenne; Lucie Planche; Marie-Christine Bene; Berthe-Marie Imbert-Marcille
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-16
Journal Detail:
Title:  Transfusion     Volume:  -     ISSN:  1537-2995     ISO Abbreviation:  Transfusion     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 American Association of Blood Banks.
Affiliation:
Service d'Hématologie Clinique, CHU Hôtel Dieu, Université de Nantes, Centre d'Investigation Clinique en Cancerologie (CI2C) and INSERM U892; Service d'Hematologie/Biologie, Service de Gynecologie/Obstetrique; Cellule de Promotion à la Recherche Clinique, CHU de Nantes; Service de Virologie, CHU de Nantes, and UPRES-EA4271, PRES L'UNAM, Nantes University; Unité d'Ingénierie Cellulaire, EFS Pays de la Loire, Nantes, France.
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