| Characterization of a variant of the polysaccharide acetan produced by a mutant of Acetobacter xylinum strain CR1/4. | |
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MedLine Citation:
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PMID: 8444650 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Acetobacter xylinum NRRL B42 (NCIB 40123) produces both cellulose and a complex anionic branched heteropolysaccharide called acetan. Chemical mutagenesis was used to isolate stable cellulose-minus Acetobacter xylinum mutants. Further chemical mutagenesis of these cellulose-minus A. xylinum bacteria was used to select mutants which secrete polysaccharides which are variants of the acetan structure. Preparation, purification and characterization of these polysaccharides are described. Methylation analysis of the polysaccharide structure CR1/4 suggests that the polysaccharide has an acetan structure with a truncated sidechain terminating in glucuronic acid. |
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Authors:
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C A MacCormick; J E Harris; A P Gunning; V J Morris |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of applied bacteriology Volume: 74 ISSN: 0021-8847 ISO Abbreviation: J. Appl. Bacteriol. Publication Date: 1993 Feb |
Date Detail:
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Created Date: 1993-04-06 Completed Date: 1993-04-06 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7503050 Medline TA: J Appl Bacteriol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 196-9 Citation Subset: IM |
Affiliation:
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AFRC Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbohydrate Conformation Carbohydrate Sequence Carbohydrates / analysis Gluconacetobacter xylinus / genetics, isolation & purification, metabolism* Methylation Methylnitronitrosoguanidine Molecular Sequence Data Mutagenesis Polysaccharides, Bacterial / biosynthesis, chemistry*, isolation & purification |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Polysaccharides, Bacterial; 110865-71-9/acetan; 70-25-7/Methylnitronitrosoguanidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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