Document Detail


Characterization of the uterine phenotype during the peri-implantation period for LIF-null, MF1 strain mice.
MedLine Citation:
PMID:  15848385     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Leukemia inhibitory factor plays a major role in the uterus and in its absence embryos fail to implant. Our knowledge of the targets for LIF and the consequences of its absence is still very incomplete. In this study, we have examined the ultrastructure of the potential implantation site in LIF-null MF1 female mice compared to that of wild type animals. We also compared expression of proteins associated with implantation in luminal epithelium and stroma. Luminal epithelial cells (LE) of null animals failed to develop apical pinopods, had increased glycocalyx, and retained a columnar shape during the peri-implantation period. Stromal cells of LIF-null animals showed no evidence of decidual giant cell formation even by day 6 of pregnancy. A number of proteins normally expressed in decidualizing stroma did not increase in abundance in the LIF-null animals including desmin, tenascin, Cox-2, bone morphogenetic protein (BMP)-2 and -7, and Hoxa-10. In wild type animals, the IL-6 family member Oncostatin M (OSM) was found to be transiently expressed in the luminal epithelium on late day 4 and then in the stroma at the attachment site on days 5-6 of pregnancy, with a similar but not identical pattern to that of Cox-2. In the LIF-null animals, no OSM protein was detected in either LE or stroma adjacent to the embryo, indicating that expression requires uterine LIF in addition to a blastocyst signal. Fucosylated epitopes: the H-type-1 antigen and those recognized by lectins from Ulex europaeus-1 and Tetragonolobus purpureus were enhanced on apical LE on day 4 of pregnancy. H-type-1 antigen remained higher on day 5, and was not reduced even by day 6 in contrast to wild type uterus. These data point to a profound disturbance of normal luminal epithelial and stromal differentiation during early pregnancy in LIF-nulls. On this background, we also obtained less than a Mendelian ratio of null offspring suggesting developmental failure.
Authors:
A A Fouladi-Nashta; C J P Jones; N Nijjar; L Mohamet; A Smith; I Chambers; S J Kimber
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental biology     Volume:  281     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-04-25     Completed Date:  2005-07-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-21     Citation Subset:  IM    
Affiliation:
Faculty of Life Sciences, University of Manchester, 3.239 Stopford Building, Oxford Road, Manchester M13 9PT, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers
Cell Adhesion Molecules / metabolism
Embryo Implantation*
Female
Glycosylation
Interleukin-6 / genetics,  metabolism*
Lectins / metabolism
Leukemia Inhibitory Factor
Male
Mice
Mice, Knockout
Phenotype
Pregnancy
Uterus / metabolism*,  ultrastructure*
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Cell Adhesion Molecules; 0/Interleukin-6; 0/Lectins; 0/Leukemia Inhibitory Factor; 0/Lif protein, mouse

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