Document Detail


Characterization of uronic-acid-rich inhibitor of calcium oxalate crystallization isolated from rat urine.
MedLine Citation:
PMID:  7676539     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human urine contains several macromolecules which inhibit calcium oxalate crystallization. Uronic-acid-rich protein (UAP), a glycoprotein with a molecular weight of approximately 35 kDa, is one such inhibitor. Here we report the characterization of UAP extracted from rat urine using three chromatographic steps including diethylaminoethanol (DEAE)-Sephacel, Sephacryl S-300 and Mono Q column and compare it with human UAP. The molecular weight of rat UAP (UAPr) is similar to that of human UAP (UAPh), being approximately 35 kDa as estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Their amino acid compositions are identical, they contain a high percentage of aspartic and glutamic acids and they react positively in the carbazole reaction, suggesting that they contain uronic acid. The inhibitory activities of UAPh and UAPr were assayed on a calcium oxalate crystallization system in vitro using [45Ca]calcium chloride. Both exert a strong inhibition, suggesting that UAPr, like UAPh, plays an important role in preventing and reducing calcium oxalate crystallization in the urine. On Western blot analysis, both UAPh and UAPr immunoreact with inter-alpha-trypsin inhibitor (ITI) antibody. Nevertheless, using the Ouchterlony immunodiffusion technique, there was no precipitation line between ITI antibody and UAP. Therefore, we hypothesize that UAP is related to ITI and that they may have the same epitope but are not completely identical. We conclude that UAP belongs to the ITI superfamily of macromolecules which contribute to the regulation of the calcium oxalate crystallization process.
Authors:
F Atmani; S R Khan
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Urological research     Volume:  23     ISSN:  0300-5623     ISO Abbreviation:  Urol. Res.     Publication Date:  1995  
Date Detail:
Created Date:  1995-10-19     Completed Date:  1995-10-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0364311     Medline TA:  Urol Res     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  95-101     Citation Subset:  IM    
Affiliation:
University of Florida, College of Medicine, Department of Pathology and Laboratory Medicine, Gainesville 32610, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Calcium Oxalate / antagonists & inhibitors*
Chromatography / methods
Crystallization
Humans
Male
Molecular Sequence Data
Peptide Fragments / genetics
Rats
Rats, Sprague-Dawley
Sequence Homology, Amino Acid
Grant Support
ID/Acronym/Agency:
P01DK20586/DK/NIDDK NIH HHS; R01DK41434/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Peptide Fragments; 25454-23-3/Calcium Oxalate

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