Document Detail


Characterization of a unique sigma54-dependent PTS operon of the lactose family in Listeria monocytogenes.
MedLine Citation:
PMID:  14505817     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The sigma(54) subunit of the RNA polymerase directs the expression of specific operons in association with cognate activators. Three different activators have been detected in the Listeria monocytogenes genome on the basis of the high conservation of a specific domain. Among them, the LacR activator, of the LevR family, was found just upstream from a newly described sigma(54)-dependent operon, lpo, which presents a classical -24/-12 consensus promoter. The lpo operon encodes proteins similar to subunits of a PTS permease (EII) of the lactose family, namely LpoA (IIA) and LpoB (IIB). It also encodes a third putative protein, LpoO, with an unknown function but sharing high similarity with proteins also encoded within PTS operons from other bacteria and bearing a RGD motif. The expression of lpo was clearly dependent on LacR and sigma(54), and was induced by cellobiose, chitobiose and lactose. It underlies that the lpo operon likely encodes proteins involved in the utilization of these sugars by L. monocytogenes.
Authors:
Karine Dalet; Safia Arous; Yves Cenatiempo; Yann Héchard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimie     Volume:  85     ISSN:  0300-9084     ISO Abbreviation:  Biochimie     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-09-24     Completed Date:  2004-06-01     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  France    
Other Details:
Languages:  eng     Pagination:  633-8     Citation Subset:  IM    
Affiliation:
Laboratoire de Microbiologie Fondamentale et Appliquée, IBMIG, UFR Sciences, ESA CNRS 6031, Université de Poitiers, 40, avenue du Recteur Pineau, 86022 cedex, Poitiers, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / genetics,  physiology
Base Sequence
Carbohydrate Metabolism*
Cellobiose / pharmacology
DNA-Binding Proteins*
DNA-Directed RNA Polymerases / physiology*
Disaccharides / pharmacology
Enhancer Elements, Genetic / genetics,  physiology
Lac Repressors
Lactose / pharmacology
Listeria monocytogenes / enzymology,  genetics*
Molecular Sequence Data
Operon / drug effects,  genetics*
Phosphoenolpyruvate Sugar Phosphotransferase System / chemistry,  drug effects,  genetics*
RNA Polymerase Sigma 54
Repressor Proteins / genetics,  physiology
Sigma Factor / physiology*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA-Binding Proteins; 0/Disaccharides; 0/Lac Repressors; 0/Repressor Proteins; 0/Sigma Factor; 16462-44-5/Cellobiose; 577-76-4/chitobiose; 63-42-3/Lactose; EC 2.7.1.-/Phosphoenolpyruvate Sugar Phosphotransferase System; EC 2.7.7.6/DNA-Directed RNA Polymerases; EC 2.7.7.6/RNA Polymerase Sigma 54

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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