| Characterization of two tropomyosin isoforms from the fast skeletal muscle of bluefin tuna Thunnus thynnusorientalis. | |
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MedLine Citation:
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PMID: 20646991 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fast skeletal muscle tropomyosin (TM) of tunas is composed of nearly equimolar amount of two isoforms designated alpha-TM and beta-TM expediently based on their migration behavior in SDS-PAGE, whereas corresponding TMs from the other fish species are homogenous (alpha-type). The presence of beta-TM is thus specific to tunas so far. The amino acid sequence of beta-TM from bluefin tuna Thunnus thynnus orientalis, which has not been revealed to date unlike alpha-TM, was successfully obtained in this study by cDNA cloning. The coding region of beta-TM cDNA comprised of an open reading frame of 855bp encoding 284 amino acid residues, like most of the TMs. Unexpectedly, the sequence of beta-TM showed high similarity to those of other vertebrate alpha-type TMs including tuna alpha-TM. Phylogenetic analysis also showed that beta-TM has the closest relationship with alpha-TM of tuna. This fact was quite unlike the relation of mammalian alpha- and beta-TMs. Based on the distribution of amino acid substitutions, it was suggested that tuna TM isoforms are the products of different genes. By thermodynamic analysis of native and reconstituted TMs, it was demonstrated that beta-TM is less thermostable than alpha-TM. Proteolytic digestion also supported the lower stability of the former. |
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Authors:
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Yoshihiro Ochiai; Hideo Ozawa; Ming-Chih Huang; Shugo Watabe |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-18 |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 502 ISSN: 1096-0384 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-09 Completed Date: 2010-10-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: United States |
Other Details:
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Languages: eng Pagination: 96-103 Citation Subset: IM |
Copyright Information:
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2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Aquatic Bioscience, The University of Tokyo, Bunkyo, Japan. aochiai@mail.ecc.u-tokyo.ac.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence DNA, Complementary / genetics, metabolism Electrophoresis, Polyacrylamide Gel Fishes / genetics, metabolism Muscle, Skeletal / metabolism* Perciformes / genetics, metabolism Protein Isoforms / genetics, metabolism Tropomyosin / chemistry*, genetics, metabolism Tuna / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Protein Isoforms; 0/Tropomyosin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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