Document Detail


Characterization of two scleroderma autoimmune antigens that copurify with human ribonuclease P.
MedLine Citation:
PMID:  9037013     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human RNase P has been purified more than 2000-fold from HeLa cells. In addition to the RNA component, H1 RNA, polypeptides of molecular masses 14, 20, 25, 30, 38, and 40 kDa copurify with the enzyme activity. Sera from two different patients with the autoimmune disease scleroderma were used to immunodeplete human RNase P activity. These same sera cross-reacted on immunoblots with two of the copurifying polypeptides, p30 and p38, whereas an autoimmune serum that does not immunodeplete RNase P activity did not react with these proteins. Peptide fragments derived from purified p30 and p38 facilitated the molecular cloning and sequencing of cDNAs coding for these two polypeptides, which are now designated as Rpp30 and Rpp38, respectively. RPP38 cDNA encodes a polypeptide that may be identical to a previously identified antigen of approximately 40 kDa, which is immunoprecipitated by Th and To autoimmune antisera, and that has been implicated as a protein subunit of human RNase P by virtue of its ability to bind to H1 RNA in vitro. The second autoimmune antigen, Rpp30, as such, has not been described previously.
Authors:
P S Eder; R Kekuda; V Stolc; S Altman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  94     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-03-27     Completed Date:  1997-03-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1101-6     Citation Subset:  IM    
Affiliation:
Department of Biology, Yale University, New Haven, CT 06520, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U77664;  U77665
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Autoantigens / genetics*,  immunology,  isolation & purification
Base Sequence
Cloning, Molecular
Cross Reactions
Endoribonucleases / chemistry*,  immunology
Hela Cells
Humans
Immunoblotting
Molecular Sequence Data
Precipitin Tests
RNA, Catalytic / chemistry*,  immunology
Ribonuclease P
Scleroderma, Systemic / immunology*
Sequence Analysis, DNA
Grant Support
ID/Acronym/Agency:
GM-19422/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Autoantigens; 0/RNA, Catalytic; 0/RPP30 protein, human; EC 3.1.-/Endoribonucleases; EC 3.1.26.5/RPP14 protein, human; EC 3.1.26.5/RPP38 protein, human; EC 3.1.26.5/Ribonuclease P
Comments/Corrections

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