Document Detail

Characterization of two novel pre-B-cell lines (LK63 and LiLa-1): potential models of pre-B-cell differentiation.
MedLine Citation:
PMID:  1378917     Owner:  NLM     Status:  MEDLINE    
In this report we describe two newly isolated pre-B acute lymphoblastic leukaemia cell lines. Both cell lines lack EBV as detected by the EBNA-1 gene probed Southern-blots. Neither cell line expressed the B-cell-specific CD20 antigen on the cell membrane. However surface expression of CD20 was induced by phorbol ester (TPA) on both LiLa-1 and LK63 cell lines. Other pre-B and B-cell lines, such as Reh, Nalm-1, and BALL-1 did not exhibit these changes in phenotype. Previous immunoprecipitation studies have noted that a broad 50-55 kD band co-precipitates with the characteristic 33-37 kD CD20 protein. We demonstrate that, while the 33-37 kD CD20 species was undetectable on resting LiLa-1 and LK63 cells, in each case a 50-55 kD protein was immunoprecipitated by the CD20 antibody. However, the failure to detect any cell surface CD20-associated antigen on the control cells by immunophenotyping indicated that the CD20 epitope of the 50-55 kD molecule was not expressed on the cell surface. Following exposure to TPA the 50-55 kD species was reduced over 48-72 h while the level of the p33-37 CD20 protein was increased. Northern-blot analysis showed that the 50-55 kD protein was not a cryptic form of CD20 as the uninduced cells contained no detectable CD20 mRNA. The decrease of the 50-55 kD protein and the acquisition of the mature CD20 molecule were paralleled by a decline in proliferative activity in both cell lines. As expression of CD20 by normal pre-B cells also coincides with the cessation of cell division and maturation towards a mature B-cell phenotype, these cell lines appear to represent models for a discrete stage of B-cell differentiation which may be valuable in defining the signals regulating pre-B-cell proliferation.
E Salvaris; J R Novotny; K Welch; L Campbell; A W Boyd
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Leukemia research     Volume:  16     ISSN:  0145-2126     ISO Abbreviation:  Leuk. Res.     Publication Date:    1992 Jun-Jul
Date Detail:
Created Date:  1992-08-21     Completed Date:  1992-08-21     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7706787     Medline TA:  Leuk Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  655-63     Citation Subset:  IM    
Lions Clinical Cancer Research Laboratory, Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia.
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MeSH Terms
Antigens, CD / metabolism
Antigens, CD20
Antigens, Differentiation, B-Lymphocyte / metabolism
B-Lymphocytes / immunology,  metabolism,  pathology
Blotting, Northern
Blotting, Southern
Cell Cycle / drug effects
Cell Differentiation / drug effects
Cell Line
Membrane Proteins / metabolism
Models, Biological
Neoplasm Proteins / metabolism
Precipitin Tests
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / immunology,  metabolism,  pathology*
Tetradecanoylphorbol Acetate / pharmacology
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD20; 0/Antigens, Differentiation, B-Lymphocyte; 0/Membrane Proteins; 0/Neoplasm Proteins; 16561-29-8/Tetradecanoylphorbol Acetate

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