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Characterization of two novel cold-inducible K(3) dehydrin genes from alfalfa (Medicago sativa spp. sativa L.).
MedLine Citation:
PMID:  23188214     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Dehydrin defines a complex family of intrinsically disordered proteins with potential adaptive value with regard to freeze-induced cell dehydration. Search within an expressed sequence tags library from cDNAs of cold-acclimated crowns of alfalfa (Medicago sativa spp. sativa L.) identified transcripts putatively encoding K(3)-type dehydrins. Analysis of full-length coding sequences unveiled two highly homologous sequence variants, K(3)-A and K(3)-B. An increase in the frequency of genotypes yielding positive genomic amplification of the K(3)-dehydrin variants in response to selection for superior tolerance to freezing and the induction of their expression at low temperature strongly support a link with cold adaptation. The presence of multiple allelic forms within single genotypes and independent segregation indicate that the two K(3) dehydrin variants are encoded by distinct genes located at unlinked loci. The co-inheritance of the K(3)-A dehydrin with a Y(2)K(4) dehydrin restriction fragment length polymorphism with a demonstrated impact on freezing tolerance suggests the presence of a genome domain where these functionally related genes are located. These results provide additional evidence that dehydrin play important roles with regard to tolerance to subfreezing temperatures. They also underscore the value of recurrent selection to help identify variants within a large multigene family in allopolyploid species like alfalfa.
Authors:
Marie-Pier Dubé; Yves Castonguay; Jean Cloutier; Josée Michaud; Annick Bertrand
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-28
Journal Detail:
Title:  TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik     Volume:  -     ISSN:  1432-2242     ISO Abbreviation:  Theor. Appl. Genet.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0145600     Medline TA:  Theor Appl Genet     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Crops and Soils Research and Development Center, Agriculture and Agri-Food Canada, 2560 Hochelaga Blvd., Quebec, QC, G1V 2J3, Canada.
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