Document Detail


Characterization of two newly established cell lines derived from squamous cell carcinomas of the oesophagus.
MedLine Citation:
PMID:  9216685     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study describes characteristics of two newly established cell lines (OSC-1 and OSC-2), derived from two oesophageal squamous cell carcinomas. Morphologically, OSC-1 cells and OSC-2 cells grew in epithelial cobblestone patterns with cells piling up to 4 cells. Ultrastructurally, both cell lines showed formation of desmosomes; however, tonofilaments were only formed by OSC-2 cells. Immunohistochemical investigations revealed coexpression of vimentin and cytokeratin in OSC-1 cells and OSC-2 cells. A cytokeratin subtype typical for mature squamous epithelia (cytokeratin 13) was expressed only in OSC-2 cells. OSC-1 cells showed tumour formation in nude mice, whereas OSC-2 cells did not. Cytogenetic analysis revealed that OSC-1 cells had a hyperdiploid karyotype and OSC-2 cells had a near-triploid karyotype. In both cell lines, the formation of multicellular spheroids could be induced. In conclusion, in comparison with OSC-2 cells the OSC-1 cells were characterized by a poorer degree of differentiation and by a more aggressive growth behaviour in vivo.
Authors:
M Sarbia; N Bösing; B Hildebrandt; P Koldovsky; C D Gerharz; H E Gabbert
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  17     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    1997 May-Jun
Date Detail:
Created Date:  1997-08-04     Completed Date:  1997-08-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  2185-92     Citation Subset:  IM    
Affiliation:
Institute of Pathology, Heinrich-Heine-University, Düsseldorf, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma, Squamous Cell / genetics,  pathology*,  ultrastructure
Cell Culture Techniques / methods
Cell Cycle
Cell Line
Chromosome Banding
Desmosomes / pathology,  ultrastructure
Esophageal Neoplasms / genetics,  pathology*,  ultrastructure
Humans
Immunohistochemistry
Karyotyping
Keratins / analysis,  biosynthesis
Metaphase
Mice
Mice, Nude
Microscopy, Electron
Microscopy, Phase-Contrast
Transplantation, Heterologous
Tumor Cells, Cultured
Vimentin / analysis,  biosynthesis
Chemical
Reg. No./Substance:
0/Vimentin; 68238-35-7/Keratins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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