Document Detail

Characterization of two hemorrhagic factors isolated from the venom of Bothrops neuwiedi (jararaca pintada).
MedLine Citation:
PMID:  6427978     Owner:  NLM     Status:  MEDLINE    
Two hemorrhagic factors were isolated from the venom of Bothrops neuwiedi (jararaca pintada) by ammonium sulfate precipitation followed by chromatography on DEAE-Sephadex A-50 and DEAE-cellulose DE-32, gel filtration on Sephadex G-100 and polyacrylamide-gel electrophoresis. These factors were named neuwiedi hemorrhagic factors NHFa and NHFb. They are acidic proteins of pI 4.2-4.3 and consist of single polypeptide chains of molecular weights 46,000 and 58,000, respectively, as determined by sodium dodecyl sulfate polyacrylamide-gel electrophoresis. The hemorrhagic activity of NHFb is 23 times stronger than that of NHFa. Both hydrolyse casein, although NHFa is about 20 times more active than NHFb. They are metalloproteins inhibited by EDTA and 1,10-phenanthroline. NHFa and NHFb are serologically closely related antigens. These two factors are recognized as identical antigens by horse serum against crude Bothrops neuwiedi venom. However, the rabbit specific antiserum was able to differentiate NHFa from NHFb showing, nevertheless, that they have common determinants apart from specific determinants for each one.
F R Mandelbaum; M T Assakura; A P Reichl
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  22     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  1984  
Date Detail:
Created Date:  1984-07-02     Completed Date:  1984-07-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  193-206     Citation Subset:  IM    
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MeSH Terms
Blood Coagulation / drug effects
Carboxylic Ester Hydrolases / analysis
Crotalid Venoms / isolation & purification*,  toxicity
Electrophoresis, Polyacrylamide Gel / methods
Hemorrhage / chemically induced
Isoelectric Focusing
Molecular Weight
Peptide Hydrolases / analysis
Proteins / analysis
Reg. No./Substance:
0/Caseins; 0/Crotalid Venoms; 0/Proteins; 0/crotalid hemorrhagic factor B; 90881-48-4/crotalid hemorrhagic factor A; EC 3.1.-/arginine esterase; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.4.-/Peptide Hydrolases

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