Document Detail


Characterization of tumor microvascular structure and permeability: comparison between magnetic resonance imaging and intravital confocal imaging.
MedLine Citation:
PMID:  20615006     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Solid tumors are characterized by abnormal blood vessel organization, structure, and function. These abnormalities give rise to enhanced vascular permeability and may predict therapeutic responses. The permeability and architecture of the microvasculature in human osteosarcoma tumors growing in dorsal window chambers in athymic mice were measured by confocal laser scanning microscopy (CLSM) and dynamic contrast enhanced magnetic resonance imaging (DCE-MRI). Dextran (40 kDa) and Gadomer were used as molecular tracers for CLSM and DCE-MRI, respectively. A significant correlation was found between permeability indicators. The extravasation rate K(i) as measured by CLSM correlated positively with DCE-MRI parameters, such as the volume transfer constant K(trans) and the initial slope of the contrast agent concentration-time curve. This demonstrates that these two techniques give complementary information. Extravasation was further related to microvascular structure and was found to correlate with the fractal dimension and vascular density. The structural parameter values that were obtained from CLSM images were higher for abnormal tumor vasculature than for normal vessels.
Authors:
Nina Kristine Reitan; Marte Thuen; Pål Erik Goa; Catharina de Lange Davies
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  15     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:    2010 May-Jun
Date Detail:
Created Date:  2010-07-09     Completed Date:  2010-10-29     Revised Date:  2011-08-01    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  036004     Citation Subset:  IM    
Affiliation:
Norwegian University of Science and Technology, Department of Physics, Hogskoleringen 5, N-7491 Trondheim, Norway. nina.reitan@ntnu.no
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Neoplasms / blood supply*
Capillary Permeability
Contrast Media
Disease Models, Animal
Female
Humans
Linear Models
Magnetic Resonance Imaging / methods*
Mice
Mice, Inbred BALB C
Mice, Nude
Microscopy, Confocal / methods*
Microvessels / metabolism*,  pathology*
Neovascularization, Pathologic / pathology
Osteosarcoma / blood supply*
Structure-Activity Relationship
Transplantation, Heterologous
Chemical
Reg. No./Substance:
0/Contrast Media
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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