Document Detail


Characterization of a tuberculosis patient's sera reactive mycobacterium tuberculosis transcription factor rv2175c.
MedLine Citation:
PMID:  24295180     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The transcriptional factor plays an important role in the regulation of gene expression and crucial to understand the biology of organisms. Mycobacterium tuberculosis, the causative agent of tuberculosis, harbors multiple transcriptional factors. Few transcriptional factors are well-documented antigens. Based on the screening of antigen with TB patients sera, a functional unknown ORF (Rv2175c) annotated as transcriptional factor was heterologously expressed, purified and for serodiagnostic value. E.coli recombinant Rv2175c protein was produced and antibodies were generated in rabbit using the recombinant antigens. ELISA and novel electrochemical immunosensor were employed in a parallel fashion in order to define its serodiagnositic value. Electrochemical immunosensor showed a sharp difference between health and TB sera. The data showed that Rv2715c is antigenic and can be used for TB seradignosis candidate in non BCG vaccinated areas.
Authors:
Peifu Zhou; Yali Yuan; Qinglin Du; Ruo Yuan; Jianping Xie
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunoassay & immunochemistry     Volume:  35     ISSN:  1532-4230     ISO Abbreviation:  J Immunoassay Immunochem     Publication Date:  2014 Apr 
Date Detail:
Created Date:  2013-12-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100963688     Medline TA:  J Immunoassay Immunochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  173-82     Citation Subset:  IM    
Affiliation:
a Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University , Chongqing , China.
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