Document Detail


Characterization of the transport mechanism and permeant binding profile of the uridine permease Fui1p of Saccharomyces cerevisiae.
MedLine Citation:
PMID:  16854981     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The uptake of Urd into the yeast Saccharomyces cerevisiae is mediated by Fui1p, a Urd-specific nucleoside transporter encoded by the FUI1 gene and a member of the yeast Fur permease family, which also includes the uracil, allantoin, and thiamine permeases. When Fui1p was produced in a double-permease knock-out strain (fur4Deltafui1Delta) of yeast, Urd uptake was stimulated at acidic pH and sensitive to the protonophore carbonyl cyanide m-chlorophenylhydrazone. Electrophysiological analysis of recombinant Fui1p produced in Xenopus oocytes demonstrated that Fui1p-mediated Urd uptake was dependent on proton cotransport with a 1:1 stoichiometry. Mutagenesis analysis of three charged amino acids (Glu(259), Lys(288), and Asp(474) in putative transmembrane segments 3, 4, and 7, respectively) revealed that only Lys(288) was required for maintaining high Urd transport efficiency. Analysis of binding energies between Fui1p and different Urd analogs indicated that Fuip1 interacted with C(3')-OH, C(2')-OH, C(5)-H, and N(3)-H of Urd. Fui1p-mediated transport of Urd was inhibited by analogs with modifications at C-5', but was not inhibited significantly by analogs with modifications at C-3', C-5, and N-3 or inversions of configuration at C-2' and C-3'. This characterization of Fui1p contributes to the emerging knowledge of the structure and function of the Fur family of permeases, including the Fui1p orthologs of pathogenic fungi.
Authors:
Jing Zhang; Kyla M Smith; Tracey Tackaberry; Xuejun Sun; Pat Carpenter; Melissa D Slugoski; Morris J Robins; Lars P C Nielsen; Ireneusz Nowak; Stephen A Baldwin; James D Young; Carol E Cass
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-07-19
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  281     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-18     Completed Date:  2006-11-02     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  28210-21     Citation Subset:  IM    
Affiliation:
Membrane Protein Research Group and the Department of Oncology, University of Alberta, Alberta T6H 1Z2, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Hydrogen-Ion Concentration
Membrane Transport Proteins / chemistry,  metabolism*
Nucleoside Transport Proteins
Protein Transport
Saccharomyces cerevisiae Proteins / antagonists & inhibitors,  chemistry,  metabolism*
Structure-Activity Relationship
Uridine / metabolism
Xenopus
Chemical
Reg. No./Substance:
0/FUI1 protein, S cerevisiae; 0/Membrane Transport Proteins; 0/Nucleoside Transport Proteins; 0/Saccharomyces cerevisiae Proteins; 58-96-8/Uridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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