Document Detail

Characterization of transcriptional oscillation of an Arabidopsis homolog of PnC401 related to photoperiodic induction of flowering in Pharbitis nil.
MedLine Citation:
PMID:  14988494     Owner:  NLM     Status:  MEDLINE    
AtC401 is an Arabidopsis homolog of PnC401 that is related to photoperiodic induction of flowering in Pharbitis nil. These genes show free-running rhythms. To study the free-running rhythm of AtC401, we fused a firefly luciferase reporter to the AtC401 promoter and transformed it into Arabidopsis plants. The observed bioluminescence oscillated under continuous light and continuous dark only with sucrose supplementation. The free-running period of bioluminescence was temperature-compensated between 22 degrees C and 30 degrees C. Light-pulse experiments under continuous darkness produced a phase-response curve typical of circadian rhythms. We conclude that rhythmic expression of AtC401 is controlled by a circadian oscillator.
Taichi Oguchi; Kimiyo Sage-Ono; Hiroshi Kamada; Michiyuki Ono
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant & cell physiology     Volume:  45     ISSN:  0032-0781     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-02-27     Completed Date:  2004-06-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  232-5     Citation Subset:  IM    
Gene Research Center, Institute of Biological Sciences, University of Tsukuba, Tsukuba, 305-8572 Japan.
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MeSH Terms
Arabidopsis / genetics*,  growth & development,  radiation effects
Arabidopsis Proteins / genetics*
Biological Clocks / genetics*
Circadian Rhythm / genetics
Flowers / genetics*,  growth & development,  radiation effects
Gene Expression Regulation, Plant / genetics
Genes, Regulator / genetics*
Genes, Reporter / genetics
Ipomoea / genetics*
Luciferases / genetics
Luminescent Measurements
Photic Stimulation
Plants, Genetically Modified / genetics,  growth & development,  radiation effects
Reg. No./Substance:
0/Arabidopsis Proteins; EC 1.13.12.-/Luciferases

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