| Characterization of toxigenic vibrios isolated from the freshwater environment of Hiroshima, Japan. | |
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MedLine Citation:
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PMID: 2690736 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The occurrence and characterization of toxigenic vibrios in surface water and sediment samples of the fresh water environment of the Ohta River were studied. The membrane filter, pad preenrichment technique, followed by the placement of membranes onto thiosulfate citrate-bile salt-sucrose agar, was used for the enumeration of total vibrios. Qualitative examination of pathogenic vibrios was also attempted. In addition, a survey was conducted to determine the incidence of Clostridium botulinum in sediment samples of the Ohta River and the Hiroshima coast. In the identification of 361 strains, 12 species of Vibrio and two species of Listonella were observed. Non-01 Vibrio cholerae was prevalent among the members of the genus Vibrio. Vibrio parahaemolyticus (serotype 04:K34), isolated in fresh water, is significant and suggests that some still unknown conditions promote the survival of these organisms in fresh water. An estimated 132 strains were hemolytic by a simple agar method, and further characterization revealed that 82% of the hemolytic vibrios (107 strains) produced various toxins. About 71% (93 strains) elaborated cytotoxin, 55% (72 strains) produced hemolysin, and 44% (58 strains) responded for both cytotoxin and hemolysin in the crude toxin extracts. All the non-01 V. cholerae showed cytotoxic activity, and the virulent strains of Vibrio fluvialis and Vibrio spp. showed cytotonic responses in RK-13 cells. Of 36 sediment samples tested, 10 harbored C. botulinum spores (28%) and were isolated invariably in all the regions of the Hiroshima coast and in the Ohta River, except the upper region of the Ohta River. |
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Authors:
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K Venkateswaran; C Kiiyukia; M Takaki; H Nakano; H Matsuda; H Kawakami; H Hashimoto |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 55 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 1989 Oct |
Date Detail:
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Created Date: 1990-02-02 Completed Date: 1990-02-02 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2613-8 Citation Subset: IM |
Affiliation:
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Department of Food Microbiology and Hygiene, Faculty of Applied Biological Science, Hiroshima University, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Toxins
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biosynthesis Clostridium botulinum / isolation & purification Cytotoxins / analysis, biosynthesis Fresh Water Hemolysin Proteins / biosynthesis Hemolysis Japan Micropore Filters Serotyping Spores, Bacterial / isolation & purification Vibrio / isolation & purification*, metabolism Vibrio cholerae / isolation & purification, metabolism Vibrio parahaemolyticus / isolation & purification, metabolism Water Microbiology* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Toxins; 0/Cytotoxins; 0/Hemolysin Proteins |
| Comments/Corrections | |
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