| Characterization of thrombomodulin expression in response to retinoic acid and identification of a retinoic acid response element in the human thrombomodulin gene. | |
| | |
MedLine Citation:
|
PMID: 8207015 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Thrombomodulin (TM) is an essential cofactor for the physiologic activation of the anticoagulant protein C by thrombin. We have observed that the expression of TM mRNA in response to retinoic acid was markedly increased in human U937 monoblast-like cells, and human MEG01 megakaryocyte-like cells, but not in human umbilical vein cells, murine hemangioma cells, human K562 erythroblast-like cells, and murine HSD fibroblast-like cells. TM activity in U937 cells and MEG01 cells was not detectable in untreated cells, but developed rapidly after treatment with retinoic acid. In endothelial cells there was minimal change in TM activity in response to retinoic acid treatment. We have isolated clones for the genes for murine and human TM and have identified potential retinoic acid response elements in the 5'-flanking region of the human gene. In U937 cells the increase in mRNA levels was associated with increased transcription, and transient transfection studies with reporter plasmids demonstrate functional retinoic acid response elements present in the 5'-flanking region of the gene. Deletion of, and mutations introduced into, the potential retinoic acid response element confirm the functional response in transient transfections. |
| | |
Authors:
|
W A Dittman; S C Nelson; P K Greer; E T Horton; M L Palomba; S S McCachren |
Related Documents
:
|
21262985 - Ethanolic and aqueous extracts derived from australian fungi inhibit cancer cell growth... 15054865 - Olf/ebf proteins are expressed in neuroblastoma cells: potential regulators of the chro... 2045425 - Expression and function of interleukin-6 in epithelial cells. 8912865 - The nad(p)h:quinone oxidoreductase locus in human colon carcinoma hct 116 cells resista... 1558765 - Structural and functional relationships among the rtx toxin determinants of gram-negati... 6582925 - Relationship between the per cent of marrow cells in s phase and the outcome of remissi... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 269 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1994 Jun |
Date Detail:
|
Created Date: 1994-07-14 Completed Date: 1994-07-14 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 16925-32 Citation Subset: IM |
Affiliation:
|
Division of Hematology/Oncology, Durham Veterans Administration Medical Center, North Carolina. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/M74231; M74564 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Base Sequence Binding Sites CHO Cells Cell Line Cricetinae DNA / genetics, metabolism Hemangioma / metabolism Hominidae / genetics* Humans Megakaryocytes / metabolism Mice Molecular Sequence Data Promoter Regions, Genetic* / drug effects Thrombomodulin / biosynthesis*, genetics* Transcription, Genetic Transfection Tretinoin / pharmacology* Tumor Cells, Cultured Umbilical Veins / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
P50-AR39162/AR/NIAMS NIH HHS; P50-HL26309-11/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Thrombomodulin; 302-79-4/Tretinoin; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Structural analysis and functional role of the carbohydrate component of glycine transporter.
Next Document: Reversible inhibition of gene expression by a psoralen functionalized triple helix forming oligonucl...