Document Detail

Characterization of sulfation patterns of beef and pig mucosal heparins by nuclear magnetic resonance spectroscopy.
MedLine Citation:
PMID:  8737629     Owner:  NLM     Status:  MEDLINE    
Though differing only slightly in their degrees of sulfation, heparin preparations from pig mucosa and those from beef mucosa have consistently different 13C- and 1H-NMR spectra, which provide useful fingerprints for distinguishing the two types of heparin. Integrated areas of NMR signals associated with minor, undersulfated sequences (assigned by comparison with mono-dimensional spectra of selectively desulfated heparins and by analysis of two-dimensional spectra of heparins prepared from pig and beef mucosa) permit quantitation of differences in sulfation patterns. Undersulfation of pig mucosal heparins at position 6 of the hexosamine units, determined by 13C-NMR and expressed as percent glucosamines nonsulfated at C6 referred to total glucosamines, is substantially lower for pig mucosal heparins than for beef mucosal heparins (16.9-21.7% vs 36.7-40.7%; average values: 18.6% vs 40.3%). By contrast, undersulfation at position 2 of the iduronic acid units, determined by 1H-NMR and expressed as percent nonsulfated iduronic acid referred to total (sulfated + nonsulfated) iduronic acid is significantly higher for pig mucosal preparations (9.6-13.5% vs 2.1-2.7%; average values: 12.7% vs 2.3%). Pig mucosal heparins also have a significantly higher content of 3-O-sulfated glucosamine units, which are markers for the active site of heparin for antithrombin-III.
B Casu; M Guerrini; A Naggi; G Torri; L De-Ambrosi; G Boveri; S Gonella; A Cedro; L Ferró; E Lanzarotti; M Paterno; M Attolini; M G Valle
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Arzneimittel-Forschung     Volume:  46     ISSN:  0004-4172     ISO Abbreviation:  Arzneimittelforschung     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-11-08     Completed Date:  1996-11-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372660     Medline TA:  Arzneimittelforschung     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  472-7     Citation Subset:  IM    
G. Ronzoni Institute for Chemical and Biochemical Research, Milan, Italy.
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MeSH Terms
Carbohydrate Sequence
Glucosamine / chemistry,  metabolism
Heparin / chemistry*
Intestinal Mucosa / chemistry*
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Species Specificity
Sulfates / chemistry*
Reg. No./Substance:
0/Sulfates; 3416-24-8/Glucosamine; 9005-49-6/Heparin

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