| Characterization of sulfation patterns of beef and pig mucosal heparins by nuclear magnetic resonance spectroscopy. | |
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MedLine Citation:
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PMID: 8737629 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Though differing only slightly in their degrees of sulfation, heparin preparations from pig mucosa and those from beef mucosa have consistently different 13C- and 1H-NMR spectra, which provide useful fingerprints for distinguishing the two types of heparin. Integrated areas of NMR signals associated with minor, undersulfated sequences (assigned by comparison with mono-dimensional spectra of selectively desulfated heparins and by analysis of two-dimensional spectra of heparins prepared from pig and beef mucosa) permit quantitation of differences in sulfation patterns. Undersulfation of pig mucosal heparins at position 6 of the hexosamine units, determined by 13C-NMR and expressed as percent glucosamines nonsulfated at C6 referred to total glucosamines, is substantially lower for pig mucosal heparins than for beef mucosal heparins (16.9-21.7% vs 36.7-40.7%; average values: 18.6% vs 40.3%). By contrast, undersulfation at position 2 of the iduronic acid units, determined by 1H-NMR and expressed as percent nonsulfated iduronic acid referred to total (sulfated + nonsulfated) iduronic acid is significantly higher for pig mucosal preparations (9.6-13.5% vs 2.1-2.7%; average values: 12.7% vs 2.3%). Pig mucosal heparins also have a significantly higher content of 3-O-sulfated glucosamine units, which are markers for the active site of heparin for antithrombin-III. |
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Authors:
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B Casu; M Guerrini; A Naggi; G Torri; L De-Ambrosi; G Boveri; S Gonella; A Cedro; L Ferró; E Lanzarotti; M Paterno; M Attolini; M G Valle |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Arzneimittel-Forschung Volume: 46 ISSN: 0004-4172 ISO Abbreviation: Arzneimittelforschung Publication Date: 1996 May |
Date Detail:
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Created Date: 1996-11-08 Completed Date: 1996-11-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372660 Medline TA: Arzneimittelforschung Country: GERMANY |
Other Details:
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Languages: eng Pagination: 472-7 Citation Subset: IM |
Affiliation:
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G. Ronzoni Institute for Chemical and Biochemical Research, Milan, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbohydrate Sequence Cattle Glucosamine / chemistry, metabolism Heparin / chemistry* Intestinal Mucosa / chemistry* Magnetic Resonance Spectroscopy Molecular Sequence Data Species Specificity Sulfates / chemistry* Swine |
| Chemical | |
Reg. No./Substance:
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0/Sulfates; 3416-24-8/Glucosamine; 9005-49-6/Heparin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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