| Characterization of substrate specificity of a rice silicon transporter, Lsi1. | |
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MedLine Citation:
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PMID: 18214526 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lsi1 (OsNIP2;1) is the first silicon (silicic acid) transporter identified in plant, which belongs to the nodulin 26-like intrinsic membrane protein (NIP) subfamily. In this study, we characterized the function of this transporter by using the Xenopus laevis oocyte expression system. The transport activity of Lsi1 for silicic acid was significantly inhibited by HgCl2 but not by low temperature. Lsi1 also showed an efflux transport activity for silicic acid. The substrate specificity study showed that Lsi1 was able to transport urea and boric acid; however, the transport activity for silicic acid was not affected by the presence of equimolar urea and was decreased only slightly by boric acid. Furthermore, among the NIPs subgroup, OsNIP2;2 showed transport activity for silicic acid, whereas OsNIP1;1 and OsNIP3;1 did not. We propose that Lsi1 and its close homologues form a unique subgroup of NIP with a distinct ar/R selectivity filter, which is located in the narrowest region on the extra-membrane mouth and govern the substrate specificity of the pore. |
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Authors:
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Namiki Mitani; Naoki Yamaji; Jian Feng Ma |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-01-23 |
Journal Detail:
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Title: Pflügers Archiv : European journal of physiology Volume: 456 ISSN: 0031-6768 ISO Abbreviation: Pflugers Arch. Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-05-14 Completed Date: 2008-10-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0154720 Medline TA: Pflugers Arch Country: Germany |
Other Details:
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Languages: eng Pagination: 679-86 Citation Subset: IM |
Affiliation:
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Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carrier Proteins / metabolism* Genes, Plant / genetics* Oocytes / metabolism Oryza sativa / metabolism* Permeability Phosphorylation RNA, Complementary / genetics, metabolism Silicon / metabolism* Substrate Specificity Water / metabolism Xenopus laevis / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/RNA, Complementary; 7440-21-3/Silicon; 7732-18-5/Water |
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