Document Detail


Characterization of the structural and functional changes in the myocardium following focal ischemia-reperfusion injury.
MedLine Citation:
PMID:  18375718     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
High-resolution (11.7 T) cardiac magnetic resonance imaging (MRI) and histological approaches have been employed in tandem to characterize the secondary damage suffered by the murine myocardium following the initial insult caused by ischemia-reperfusion (I/R). I/R-induced changes in the myocardium were examined in five separate groups at the following time points after I/R: 1 h, day 1, day 3, day 7, and day 14. The infarct volume increased from 1 h to day 1 post-I/R. Over time, the loss of myocardial function was observed to be associated with increased infarct volume and worsened regional wall motion. In the infarct region, I/R caused a decrease in end-systolic thickness coupled with small changes in end-diastolic thickness, leading to massive wall thickening abnormalities. In addition, compromised wall thickening was also observed in left ventricular regions adjacent to the infarct region. A tight correlation (r2 = 0.85) between measured MRI and triphenyltetrazolium chloride (TTC) infarct volumes was noted. Our observation that until day 3 post-I/R the infarct size as measured by TTC staining and MRI was much larger than that of the myocyte-silent regions in trichrome- or hematoxylin-eosin-stained sections is consistent with the literature and leads to the conclusion that at such an early phase, the infarct site contains structurally intact myocytes that are functionally compromised. Over time, such affected myocytes were noted to structurally disappear, resulting in consistent infarct sizes obtained from MRI and TTC as well as trichrome and hematoxylin-eosin analyses on day 7 following I/R. Myocardial remodeling following I/R includes secondary myocyte death followed by the loss of cardiac function over time.
Authors:
Navdeep Ojha; Sashwati Roy; Jared Radtke; Orlando Simonetti; Surya Gnyawali; Jay L Zweier; Periannan Kuppusamy; Chandan K Sen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-03-28
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  294     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-09     Completed Date:  2008-08-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H2435-43     Citation Subset:  IM    
Affiliation:
Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Azo Compounds
Cell Death
Coloring Agents
Disease Models, Animal
Eosine Yellowish-(YS)
Hematoxylin
Magnetic Resonance Imaging
Male
Methyl Green
Mice
Mice, Inbred C57BL
Myocardial Infarction / complications,  pathology*,  physiopathology
Myocardial Reperfusion Injury / complications,  pathology*,  physiopathology
Myocardium / pathology*
Staining and Labeling / methods
Tetrazolium Salts
Time Factors
Ventricular Dysfunction, Left / etiology*,  pathology,  physiopathology
Ventricular Function, Left*
Ventricular Remodeling*
Grant Support
ID/Acronym/Agency:
R01-HL-073087/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Azo Compounds; 0/Coloring Agents; 0/Tetrazolium Salts; 0/trichrome stain; 17372-87-1/Eosine Yellowish-(YS); 517-28-2/Hematoxylin; 82-94-0/Methyl Green; 902-00-1/triphenyltetrazolium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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