Document Detail


Characterization of the spontaneous DNA-synthesizing and/or IgG-secreting cells in peripheral blood from patients with systemic lupus erythematosus.
MedLine Citation:
PMID:  3873305     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Characterization of the cells responsible for spontaneous DNA synthesis and/or IgG secretion in systemic lupus erythematosus (SLE) was undertaken by fractionation of the peripheral blood mononuclear cells (PBM). Each fraction was analyzed for its capacity to incorporate [3H]thymidine [( 3H]TdR) and secrete IgG without mitogen. The non-E rosette-forming cell (non-ERRC) fraction, which consisted of the surface immunoglobulin-positive [sIg(+)] cells and null cells, revealed a markedly increased spontaneous DNA synthesis (620.0 +/- 586.9 cpm) during the first hour of culture and an elevated spontaneous IgG secretion (8639 +/- 2630 ng/ml) during 9 days of culture. Of particular interest was the finding that both increased responses were conducted by the null cells; the null cell population had approximately a fourfold relative increase of [3H]TdR incorporation and a 60-fold relative increase of IgG secretion compared with the sIg(+) cell population. These results suggest that SLE patients have a small population of preactivated B-cell lineage cells, which lack sIg or have a very low density of sIg.
Authors:
K Yano; K Morimasa; T Asano; Y Shinohara; Z Ota
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical immunology and immunopathology     Volume:  35     ISSN:  0090-1229     ISO Abbreviation:  Clin. Immunol. Immunopathol.     Publication Date:  1985 Apr 
Date Detail:
Created Date:  1985-06-26     Completed Date:  1985-06-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0356637     Medline TA:  Clin Immunol Immunopathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  57-66     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antibody Formation / radiation effects
Antibody-Producing Cells*
B-Lymphocytes / metabolism
Cell Separation
Cells, Cultured
DNA / biosynthesis*
Humans
Immunoglobulin G / immunology,  secretion*
Kinetics
Lupus Erythematosus, Systemic / immunology*
Lymphocytes / radiation effects
Lymphocytes, Null / metabolism
Rosette Formation
Thymidine / metabolism
Tritium / diagnostic use
Chemical
Reg. No./Substance:
0/Immunoglobulin G; 10028-17-8/Tritium; 50-89-5/Thymidine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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