| Characterization of α-smooth muscle actin positive cells during multilineage differentiation of dental pulp stem cells. | |
| | |
MedLine Citation:
|
PMID: 22487297 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
OBJECTIVES: Dental pulp tissue contains stem cells that can differentiate into multiple lineages under specific culture conditions; the origin of these dental pulp stem cells, however, is still unknown. MATERIALS AND METHODS: Here we have utilized an α-SMA-GFP transgenic mouse model to characterize expression of a-smooth muscle actin (SMA)-GFP in subpassages of pulp-tissue-derived dental pulp cells, as perivascular cells express α-SMA. RESULTS: During subculturing, percentages of cells expressing a-SMA increased signi?cantly from passage 1 to 3. α-SMA-GFP-positive cells expanded faster than α-SMA-GFP-negative cells. The dental pulp cells at passage 3 were induced towards osteogenic, adipogenic or chondrogenic differentiation. All three differentiated cell lines expressed high levels of α-SMA (mineralized nodules, lipid droplets and chondrocyte pellets). GFP expression colocalized with differentiated osteoblasts, adipocytes and chondrocytes. Co-culturing the α-SMA-GFP-positive cells with human endothelial cells promoted formation of tube-like structures and robust vascular networks, in 3-D culture. CONCLUSIONS: Taken together, the a-SMA-GFP-positive cells were shown to have multilieange differentiation ability and to promote vascularization in a co-culture system with endothelial cells. |
| | |
Authors:
|
X Zhao; P Gong; Y Lin; J Wang; X Yang; X Cai |
Related Documents
:
|
8382687 - Inhibition of intra-golgi transport in vitro by mitotic kinase. 6733097 - Nucleoside transport in cultured mammalian cells. multiple forms with different sensiti... 2590557 - Uptake of 2',3'-dideoxyadenosine in human immunodeficiency virus-infected and noninfect... 11832427 - Mechanism and regulation of vitamin b(6) uptake by renal tubular epithelia: studies wit... 8382687 - Inhibition of intra-golgi transport in vitro by mitotic kinase. 16413417 - Activation of a novel isoform of methionine adenosyl transferase 2a and increased s-ade... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-4-8 |
Journal Detail:
|
Title: Cell proliferation Volume: - ISSN: 1365-2184 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
|
Created Date: 2012-4-10 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9105195 Medline TA: Cell Prolif Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
© 2012 Blackwell Publishing Ltd. |
Affiliation:
|
State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Clinical analysis of deep cutaneous mycoses: a 12-year experience at a single institution.
Next Document: Effects of aging on articular cartilage homeostasis.