Document Detail


Characterization of small RNAs and their target genes in wheat seedlings using sequencing-based approaches.
MedLine Citation:
PMID:  23415324     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Wheat is the most highly cultivated plant species for its grain production throughout the world. Because small RNA-dependent gene regulation is critical for successful completion of plant life cycle including its productivity, identification of not only miRNAs but also confirming their targets in wheat is important. To identify small RNAs including novel miRNAs as well as miRNA targets in wheat, we constructed small RNA and degradome libraries from wheat seedlings. Small RNA analysis resulted in identification of most conserved miRNAs including novel miRNAs that can be grouped into 32 miRNA families. The sequence analysis also led to the characterization of two abundantly expressed rRNA-derived small RNAs. To identify miRNA targets, degradome library was sequenced and the bioinformatic analysis confirmed 53 genes as targets for miRNAs and Tas3-siRNAs. Degradome analysis also confirmed a conserved fine-tuning mechanism of Tas3-siRNA abundance by siRNA-mediated silencing of TAS3 transcripts in diverse plant species. These findings added additional information to the small RNA knowledge-base in wheat.
Authors:
Yong-Fang Li; Yun Zheng; Guru Jagadeeswaran; Ramanjulu Sunkar
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-3
Journal Detail:
Title:  Plant science : an international journal of experimental plant biology     Volume:  203-204C     ISSN:  1873-2259     ISO Abbreviation:  Plant Sci.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-2-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882015     Medline TA:  Plant Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  17-24     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA.
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