| Characterization of skin abnormalities in a mouse model of osteogenesis imperfecta using high resolution magnetic resonance imaging and Fourier transform infrared imaging spectroscopy. | |
| | |
MedLine Citation:
|
PMID: 21845737 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Evaluation of the skin phenotype in osteogenesis imperfecta (OI) typically involves biochemical measurements, such as histologic or biochemical assessment of the collagen produced from biopsy-derived dermal fibroblasts. As an alternative, the current study utilized non-invasive magnetic resonance imaging (MRI) microscopy and optical spectroscopy to define biophysical characteristics of skin in an animal model of OI. MRI of skin harvested from control, homozygous oim/oim and heterozygous oim/+ mice demonstrated several differences in anatomic and biophysical properties. Fourier transform infrared imaging spectroscopy (FT-IRIS) was used to interpret observed MRI signal characteristics in terms of chemical composition. Differences between wild-type and OI mouse skin included the appearance of a collagen-depleted lower dermal layer containing prominent hair follicles in the oim/oim mice, accounting for 55% of skin thickness in these. The MRI magnetization transfer rate was lower by 50% in this layer as compared to the upper dermis, consistent with lower collagen content. The MRI transverse relaxation time, T2, was greater by 30% in the dermis of the oim/oim mice compared to controls, consistent with a more highly hydrated collagen network. Similarly, an FT-IRIS-defined measure of collagen integrity was 30% lower in the oim/oim mice. We conclude that characterization of phenotypic differences between the skin of OI and wild-type mice by MRI and FT-IRIS is feasible, and that these techniques provide powerful complementary approaches for the analysis of the skin phenotype in animal models of disease. Copyright © 2011 John Wiley & Sons, Ltd. |
| | |
Authors:
|
H C Canuto; K W Fishbein; A Huang; S B Doty; R A Herbert; J Peckham; N Pleshko; R G Spencer |
Related Documents
:
|
7800977 - Ct and pathological study on radiofrequency-induced lesion in cat thalamus. 16376187 - Ferrite-enhanced mri monitoring in hyperthermia. 10440147 - Transverse conduction capabilities of the crista terminalis in patients with atrial flu... 21427307 - Multiplanar ct and mri of collections in the retropharyngeal space: is it an abscess? 7807607 - Clinical application of spect to the localization of corticoepileptogenic focus of intr... 14985867 - Ct-guided lymphoscintigraphy in patients with squamous cell carcinoma of the head and n... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-8-15 |
Journal Detail:
|
Title: NMR in biomedicine Volume: - ISSN: 1099-1492 ISO Abbreviation: - Publication Date: 2011 Aug |
Date Detail:
|
Created Date: 2011-8-16 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8915233 Medline TA: NMR Biomed Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 John Wiley & Sons, Ltd. |
Affiliation:
|
Magnetic Resonance Imaging and Spectroscopy Section, NIH/National Institute on Aging, Intramural Research Program, GRC 4D-08 5600 Nathan Shock Drive, Baltimore, MD, USA, 21224. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Deciphering the regulatory codes in bacterial genomes.
Next Document: T(2) measurement of J-coupled metabolites in the human brain at 3T.