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Characterization of the single-strand-specific BPV-1 origin binding protein, SPSF I, as the HeLa Pur alpha factor.
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MedLine Citation:
PMID:  8759014     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
SPSF I and II are two cellular proteins which bind specifically to single-stranded DNA. SPSF I and II binding sites are found in the minimal origin of replication of BPV-1 DNA and near the P2 promoter of the cellular c-myc gene. DNA-binding properties of the two proteins to single-stranded oligonucleotides of different lengths and sequences were quantified by determination of DNA-binding constants. The binding constant of SPSF proteins to the lower strand of the BPV-1 origin was determined to be 1.5 x 10(-10) M-1. Peptide sequences derived from purified SPSF I and II revealed the identity of at least one of the SPSF proteins with the so-called HeLa Pur alpha factor. The HeLa Pur alpha factor was identified previously by virtue of its capacity to bind to purine-rich strands of the PUR element found in initiation zones of DNA replication [Bergemann, A.D., Ma,Z.-W. and Johnson, E.M. (1992) Mol. Cell. Biol. 12, 5673-5682]. Expression of the Pur cDNA confirmed the identity of the Pur alpha protein with the 42 kDa SPSF I protein. Analysis of several Pur alpha cDNA clones revealed the existence of an extended 3'-untranslated region in all Pur mRNAs.
Authors:
M Jurk; F Weissinger; F Lottspeich; U Schwarz; E L Winnacker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nucleic acids research     Volume:  24     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-09-17     Completed Date:  1996-09-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2799-806     Citation Subset:  IM    
Affiliation:
Institut für Biochemie, Ludwig-Maximilians-Universität München, Germany.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/X91648
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Bovine papillomavirus 1 / genetics*,  metabolism
Cattle
Cell Line
Cyclic AMP Response Element-Binding Protein*
DNA, Single-Stranded / metabolism
DNA-Binding Proteins / chemistry,  genetics,  isolation & purification,  metabolism*
Hela Cells
Humans
Introns
Molecular Sequence Data
Protein Binding
RNA, Messenger / genetics
Recombinant Proteins / chemistry,  genetics,  isolation & purification,  metabolism
Replication Origin
Spodoptera / cytology
Thymus Gland / metabolism
Transcription Factors
Chemical
Reg. No./Substance:
0/Cyclic AMP Response Element-Binding Protein; 0/DNA, Single-Stranded; 0/DNA-Binding Proteins; 0/PURA protein, human; 0/RNA, Messenger; 0/Recombinant Proteins; 0/Transcription Factors
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 15 Month: 7 Year: 1996
Volume: 24 Issue: 14
First Page: 2799 Last Page: 2806
ID: 146002
PubMed Id: 8759014
Publisher Item Identifier: 6b0034

Characterization of the single-strand-specific BPV-1 origin binding protein, SPSF I, as the HeLa Pur alpha factor.
M Jurk
F Weissinger
F Lottspeich
U Schwarz
E L Winnacker
Institut f?r Biochemie, Ludwig-Maximilians-Universit?t M?nchen, Germany.


Article Categories:
  • Research Article


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