| Characterization of serum interleukin-15 in healthy volunteers and patients with early arthritis to assess its potential use as a biomarker. | |
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MedLine Citation:
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PMID: 20736143 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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As interleukin-15 (IL-15) has been implicated in the pathophysiology of rheumatoid arthritis, we analysed the serum IL-15 (sIL-15) levels in healthy subjects and patients with early arthritis to establish a cut-off point that might serve to define elevated sIL-15. This is an initial step to determine whether sIL-15 has the potential for use as a biomarker for patients with early arthritis. The IL-15 concentration was measured in serum obtained from 161 healthy controls and from 174 patients with early arthritis, and the relationship between the expression of the two IL-15 mRNA variants and the sIL-15 levels was also assessed. In healthy controls, the median sIL-15 value was 0.83 [interquartile range (IQR) 0-8.68] pg/mL; there was no significant difference in the sIL-15 values according to gender [median level in males was 1.99 (IQR: 0-8.68) pg/mL and in females 0.50 (0-8.25) pg/mL: p = 0.821]. Moreover, sIL-15 levels did not correlate with age (r = 0.033, p = 0.685), and they did not display a clear circadian rhythm in healthy donors, with the median values for IL-15 close to zero at each time tested. In the light of these findings, we considered that sIL-15 was elevated if its concentration was above 20 pg/mL, since this cut-off point corresponded to the 90th percentile for this healthy population. We found that 30% of the patients with early arthritis had sIL-15 values > 20 pg/mL. The levels of sIL-15 did not correlate with disease duration in early arthritis patients, nor did they fluctuate with changes in disease activity over the follow-up period. In addition, the high level of sIL15 in patients was not associated with alterations in the alternative splicing of the IL-15 mRNA, favouring the variant that produces the protein with a long signal peptide for secretion. Serum IL-15 levels were increased in a subpopulation of patients with early arthritis, indicating that this measure may serve as a biomarker for this condition. Further studies will be necessary to determine whether the clinical evolution or response to treatment of patients with high sIL-15 levels differs. |
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Authors:
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Amalia Lamana; Ana M Ortiz; José M Alvaro-Gracia; Belén Díaz-Sánchez; Jesús Novalbos; Rosario García-Vicuña; Isidoro González-Alvaro |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-24 |
Journal Detail:
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Title: European cytokine network Volume: 21 ISSN: 1148-5493 ISO Abbreviation: Eur. Cytokine Netw. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-14 Completed Date: 2011-01-20 Revised Date: 2011-09-02 |
Medline Journal Info:
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Nlm Unique ID: 9100879 Medline TA: Eur Cytokine Netw Country: France |
Other Details:
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Languages: eng Pagination: 186-94 Citation Subset: IM |
Affiliation:
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Rheumatology Service, Hospital Universitario La Princesa, IIS La Princesa, Madrid, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Arthritis / blood* Base Sequence Biological Markers / blood* Circadian Rhythm DNA Primers Female Humans Immunoenzyme Techniques Interleukin-15 / blood*, genetics Male Middle Aged RNA, Messenger / genetics Reference Values Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/DNA Primers; 0/Interleukin-15; 0/RNA, Messenger |
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