Document Detail


Characterization of the rrn operons in the channel catfish pathogen Edwardsiella ictaluri.
MedLine Citation:
PMID:  18217940     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: To advance diagnostics and phylogenetics of Edwardsiella ictaluri by sequencing and characterizing its rrn operons. METHODS AND RESULTS: The Edw. ictaluri rrn operons were identified from a 5-7 kbp insert lambda library and from Edw. ictaluri fosmid clones. We present the complete sequences and analysis of all eight Edw. ictaluri rrn operons and unique regions located upstream and downstream. Two rrn operons were located in tandem with 169 bp separating them, which is apparently a conserved feature between Edw. ictaluri and Edwardsiella tarda. I-CeuI enzyme digestion of Edw. ictaluri genomic DNA and analysis by pulsed field gel electrophoresis indicated that rrn operon number and chromosomal locations are conserved within the species Edw. ictaluri. CONCLUSIONS: The rrn operons of Edw. ictaluri have similar structure and flanking regions compared with other members of the family Enterobacteriaceae; however, the presence of eight copies of the rrn operon makes Edw. ictaluri unique within the family. SIGNIFICANCE AND IMPACT OF THE STUDY: This research clarifies previous phylogenetic analyses of Edw. ictaluri and provides support for the Edw. ictaluri genome sequencing project. In addition, we identified a unique feature of two rrn operons that shows potential for the development of a diagnostic PCR method.
Authors:
M L Williams; G C Waldbieser; D W Dyer; A F Gillaspy; M L Lawrence
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-01-23
Journal Detail:
Title:  Journal of applied microbiology     Volume:  104     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-22     Completed Date:  2008-10-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1790-6     Citation Subset:  IM    
Affiliation:
College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
DNA Primers / genetics
Edwardsiella ictaluri / genetics*
Edwardsiella tarda / genetics
Electrophoresis, Gel, Pulsed-Field / methods
Enterobacteriaceae Infections / diagnosis
Fish Diseases / diagnosis,  microbiology
Fisheries*
Genomic Library
Ictaluridae / microbiology*
Industrial Microbiology*
Molecular Sequence Data
Polymerase Chain Reaction / methods
rRNA Operon / genetics*
Chemical
Reg. No./Substance:
0/DNA Primers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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