Document Detail


Characterization of a rice gene family encoding root-specific proteins.
MedLine Citation:
PMID:  7888615     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two cDNA clones (RCc2 and RCc3) corresponding to mRNAs highly expressed only in root tissues of rice (Oryza sativa L.) seedlings were characterized. Respectively, they encode polypeptides of 146 (14.5 kDa) and 133 amino acids (13.4 kDa) that share high (> 70%) sequence similarity with a polypeptide encoded by a cDNA (ZRP3) encoding an mRNA preferentially expressed in young maize roots. Genomic DNA blot analysis revealed that they are members of a small gene family and RCg2, the gene corresponding to RCc2, was isolated. A 1656 bp 5'-upstream sequence of RCg2 was translationally fused to a beta-glucuronidase (GUS) reporter gene and stable introduction of the chimeric construct into rice was confirmed by PCR and genomic DNA blot analyses. Histochemical analysis of transgenic rice plants containing the full-length chimeric gene showed high levels of GUS activity in mature cells and the elongation and maturation zones of primary and secondary roots, and in the root caps, but no GUS activity was detected in root meristematic regions. Surprisingly, high GUS activity was also detected in leaves of the same plants. This raises the possibility that the RCg2 5'-upstream element may not be sufficient for the proper spatial control of root specificity in transgenic rice.
Authors:
Y Xu; W G Buchholz; R T DeRose; T C Hall
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant molecular biology     Volume:  27     ISSN:  0167-4412     ISO Abbreviation:  Plant Mol. Biol.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-04-17     Completed Date:  1995-04-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9106343     Medline TA:  Plant Mol Biol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  237-48     Citation Subset:  IM    
Affiliation:
Institute of Developmental and Molecular Biology, Texas A&M University, College Station 77843-3155.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/L27208;  L27209;  L27210
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Complementary / genetics
DNA, Plant / analysis
Gene Expression Regulation, Plant*
Genes, Plant / genetics
Molecular Sequence Data
Multigene Family / genetics*
Oryza sativa / genetics*
Plant Leaves / chemistry
Plant Proteins / analysis,  genetics*
Plant Roots / chemistry,  genetics
Plants, Genetically Modified
RNA, Plant / analysis
Restriction Mapping
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/DNA, Plant; 0/Plant Proteins; 0/RCg2 protein, Oryza sativa; 0/RNA, Plant; 0/ZRP3 protein, Zea mays
Comments/Corrections
Erratum In:
Plant Mol Biol 1995 Mar;27(5):1060

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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