Document Detail


Characterization of a ribosomal protein L15 cDNA from Chironomus riparius (Diptera; Chironomidae): Transcriptional regulation by cadmium and silver nanoparticles.
MedLine Citation:
PMID:  21473924     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A full-length ribosomal protein L15 cDNA from Chironomus riparius (CrRPL15) was identified from the Expressed Sequence Tag (EST) database, and expression of the corresponding mRNA was analyzed during different developmental stages and following exposure to Cadmium chloride (Cd) and Silver nanoparticles (AgNPs). The CrRPL15 cDNA contains an open reading frame (ORF) of 615 base pairs (bp) encoding a putative protein of 204 amino acids, with a high level of sequence similarity to RPL15 proteins from other species. The consensus sequence, GXXXXGKS, which is present in other RPL15 proteins, was also present in the putative CrRPL15 protein. The CrRPL15 transcript was present at all life stages, but was most abundant in the eggs. Expression of the CrRPL15 gene was significantly down-regulated in response to exposure to both Cd and AgNPs. In contrast, expression of the heat shock protein 70 (HSP70) gene, a general stress indicator, was significantly up-regulated.
Authors:
Prakash M Gopalakrishnan Nair; Jinhee Choi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-4
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  -     ISSN:  1879-1107     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2010. Published by Elsevier Inc.
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