Document Detail

Characterization of a ribonuclease gene and encoded protein from the reptile, Iguana iguana.
MedLine Citation:
PMID:  15893436     Owner:  NLM     Status:  MEDLINE    
In this work we identify an intronless open reading frame encoding an RNase A ribonuclease from genomic DNA from the Iguana iguana IgH2 cell line. The iguana RNase is expressed primarily in pancreas, and represents the majority of the specific enzymatic activity in this tissue. The encoded sequence shares many features with its better-known mammalian counterparts including the crucial His12, Lys40 and His114 catalytic residues and efficient hydrolytic activity against yeast tRNA substrate (k(cat)/K(m)=6 x 10(4) M(-1) s(-1)), albeit at a reduced pH optimum (pH 6.0). Although the catalytic activity of the iguana RNase is not diminished by human placental RI, iguana RNase is not bactericidal nor is it cytotoxic even at micromolar concentrations. Phylogenetic analysis indicates moderate (46%) amino acid sequence similarity to a pancreatic RNase isolated from Chelydra serpentina (snapping turtle) although no specific relationship could be determined between these RNases and the pancreatic ribonucleases characterized among mammalian species. Further analysis of ribonucleases from non-mammalian vertebrate species is needed in order to define relationships and lineages within the larger RNase A gene superfamily.
Takeaki Nitto; Cynthia Lin; Kimberly D Dyer; Robert A Wagner; Helene F Rosenberg
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gene     Volume:  352     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-06-13     Completed Date:  2005-08-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  36-44     Citation Subset:  IM    
Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, United States.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cell Line
Cell Survival / drug effects
Cloning, Molecular
DNA / chemistry,  genetics
Hydrogen-Ion Concentration
Iguanas / genetics*
K562 Cells
Molecular Sequence Data
Recombinant Proteins / genetics,  metabolism
Ribonuclease, Pancreatic / genetics*,  metabolism,  pharmacology
Sequence Analysis, DNA
Reg. No./Substance:
0/Recombinant Proteins; 9007-49-2/DNA; EC, Pancreatic

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