Document Detail

Characterization of a retrovirus shuttle vector capable of either proviral integration or extrachromosomal replication in mouse cells.
MedLine Citation:
PMID:  2983188     Owner:  NLM     Status:  MEDLINE    
A retrovirus shuttle vector is described that contains the dominant selectable neo gene which confers resistance to kanamycin in bacteria and to the drug G418 in animal cells. The bacterial supF gene and the origins of DNA replication from polyomavirus and the ColE1 replicon also have been included in this vector. Infection of normal rodent cells results in single-copy proviral integration, whereas infection of mouse (MOP) cells expressing polyoma large T antigen results in extrachromosomal replication of the DNA form of the virus. The copy number of the extrachromosomal circles in MOP cells varies from 0 to 100 copies per cell. G418-resistant MOP cells lose their drug-resistant phenotype after passage under nonselective conditions, suggesting that maintenance of the extrachromosomal circles is unstable. The extrachromosomal form of the virus can be recovered as plasmids in Escherichia coli. Two-thirds of the circles analyzed were found to be structurally intact. The others have undergone rearrangements including deletions and insertions. The bacterial supF gene was found to be intact in the majority of recovered plasmids. The data presented here suggest that these retroviruses should be useful as gene transfer vectors for animal cells in culture or in vivo.
S A Berger; A Bernstein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  5     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1985 Feb 
Date Detail:
Created Date:  1985-04-11     Completed Date:  1985-04-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  305-12     Citation Subset:  IM    
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MeSH Terms
Antigens, Polyomavirus Transforming
Antigens, Viral, Tumor / genetics
DNA Replication
Drug Resistance, Microbial
Escherichia coli / genetics
Extrachromosomal Inheritance*
Genetic Vectors*
Polyomavirus / genetics
Retroviridae / genetics*
Simian virus 40 / genetics
Viral Proteins / genetics
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming; 0/Antigens, Viral, Tumor; 0/Viral Proteins

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