Document Detail


Characterization of residual lignin after SO(2)-catalyzed steam explosion and enzymatic hydrolysis of Eucalyptus viminalis wood chips.
MedLine Citation:
PMID:  10794625     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lignin component found in both water insoluble (WI) and water and alkali insoluble (WIA) fractions derived from SO(2)-impregnated steam-exploded eucalyptus chips (SEE) was isolated and characterized. Dioxane lignins with a sugar content lower than 2% (w/w) were obtained after each material was treated with commercial cellulases. The C9 formulas of both SEE-WI and SEE-WIA dioxane lignins were C(9)H(6.83)N(0.04)O(2.24)(OCH(3))(1.21)(OH(aro))(0.56)(OH(ali))(0. 77) and C(9)H(8.65)N(0.29)O(1.97)(OCH(3))(0.90)(OH(aro))(0. 46)(OH(ali))(1.02), respectively. The weight-average molecular weight (M(w)) of the SEE-WI lignin corresponded to 3.85 kDa, whereas the SEE-WIA lignin had an M(w) of 3.66 kDa for the same polydispersity of 2.4. The SEE-WIA lignin was shown to be more thermally stable than the SEE-WI lignin, requiring temperatures in the range of 520 degrees C for complete degradation. FTIR and (1)H NMR analyses of both untreated and peracetylated lignin fractions showed that (a) the alkali insoluble lignin contained a relatively higher degree of substitution in aromatic rings per C9 unit and that (b) alkaline extraction removed lignin fragments containing appreciable amounts of phenolic hydroxyl groups.
Authors:
L P Ramos; A L Mathias; F T Silva; A R Cotrim; A L Ferraz; C L Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  47     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  1999 Jun 
Date Detail:
Created Date:  2000-08-16     Completed Date:  2000-08-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2295-302     Citation Subset:  IM    
Affiliation:
Centro de Pesquisa em Química Aplicada, Departamento de Química, Universidade Federal do Paraná, Curitiba, Brazil. lramos@quimica.ufpr.br
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MeSH Terms
Descriptor/Qualifier:
Cellulase
Eucalyptus*
Hot Temperature
Hydrolysis
Lignin / analysis,  chemistry*
Plants, Medicinal*
Solubility
Spectroscopy, Fourier Transform Infrared
Sulfur Dioxide
Wood*
Chemical
Reg. No./Substance:
7446-09-5/Sulfur Dioxide; 9005-53-2/Lignin; EC 3.2.1.4/Cellulase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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