Document Detail


Characterization of the repair of injury induced by freezing Salmonella anatum.
MedLine Citation:
PMID:  4553147     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fast freezing and slow thawing of Salmonella anatum cells suspended in water resulted in injury of more than 90% of the cells that survived the treatment. The injured cells failed to form colonies on the selective medium (xyloselysine-peptone-agar with 0.2% sodium deoxycholate) but did form colonies on a nonselective (xylose-lysine-peptone-agar) plating medium. In Tryptic soy plus 0.3% yeast extract broth or minimal broth, most of the injured cells repaired within 1 to 2 hr at 25 C. Tryptic soy plus yeast extract broth supported repair to a greater extent than minimal broth. Phosphate or citrate at concentrations found in minimal broth supported repair of some cells. MgSO(4), when present with inorganic phosphate or citrate or both, increased the extent of repair. The repair process in the presence of phosphate was not prevented by actinomycin D, chloramphenicol, and D-cycloserine, but was prevented by cyanide and 2,4-dinitrophenol (only at pH 6). This suggested that the repair process might involve energy metabolism in the form of adenosine triphosphate. The freeze-injured cells were highly sensitive to lysozyme, whereas unfrozen fresh cells were not. In the presence of phosphate or minimal broth this sensitivity was greatly reduced. This suggested that, at least in some of the cells, the injury involved the lipopolysaccharide of the cell wall and adenosine triphosphate synthesis was required for repair.
Authors:
B Ray; D W Janssen; F F Busta
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied microbiology     Volume:  23     ISSN:  0003-6919     ISO Abbreviation:  Appl Microbiol     Publication Date:  1972 Apr 
Date Detail:
Created Date:  1972-06-22     Completed Date:  1972-06-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605802     Medline TA:  Appl Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  803-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / biosynthesis
Bacteriological Techniques
Cell Survival
Cell Wall / metabolism
Chloramphenicol / pharmacology
Citrates / metabolism
Culture Media
Cyanides / pharmacology
Cycloserine / pharmacology
Dactinomycin / pharmacology
Dinitrophenols / pharmacology
Freezing*
Hydrogen-Ion Concentration
Lipopolysaccharides / metabolism
Muramidase / pharmacology
Phosphates / metabolism
Salmonella* / drug effects,  growth & development,  metabolism
Time Factors
Chemical
Reg. No./Substance:
0/Citrates; 0/Culture Media; 0/Cyanides; 0/Dinitrophenols; 0/Lipopolysaccharides; 0/Phosphates; 50-76-0/Dactinomycin; 56-65-5/Adenosine Triphosphate; 56-75-7/Chloramphenicol; 68-41-7/Cycloserine; EC 3.2.1.17/Muramidase
Comments/Corrections

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