Document Detail

Characterization and relative abundance of maxi-chloride channels in Epstein-Barr virus (EBV) producer: B95-8 cells.
MedLine Citation:
PMID:  8774755     Owner:  NLM     Status:  MEDLINE    
Several Epstein-Barr virus (EBV)-transformed cell lines were used to investigate the pathogenesis of lymphoproliferative diseases and nasopharyngeal carcinoma. The studies focus on the events occurring inside the membrane. On only one occasion, the cell membrane of EBV-transformed B lymphocytes from a cystic fibrosis patient was found to express defective Cl channels (CFTR; Cystic Fibrosis Transmembrane conductance Regulator), as in the airway epithelial cell. No other type of channel in EBV-transformed cells has so far been investigated. In this study, the cell membrane of the B95-8 cell was examined by the patch-clamp technique and compared to the non-EBV-infected BJAB cell. The high conductance (approximately 300 pS) maxi-chloride (Cl) channel activity was the most frequently observed event in inside-out configurations. Under similar experimental conditions, we have found a significantly higher probability of detecting maxi-Cl channel activity on the cell membrane of B95-8 cells (69%) than on BJAB cells (27%), or as previously reported on resting murine B lymphocytes (38%) or intact human T lymphocytes (37%). The relative abundance of the maxi-Cl channel on B95-8 cells may be linked to EBV infection and/or secretory ability.
T H Yeh; M C Tsai; S Y Lee; M M Hsu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experientia     Volume:  52     ISSN:  0014-4754     ISO Abbreviation:  Experientia     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1996-10-03     Completed Date:  1996-10-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376547     Medline TA:  Experientia     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  818-26     Citation Subset:  IM    
Department of Otolaryngology, College of Medicine, National Taiwan University, Taipei.
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MeSH Terms
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / pharmacology
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / pharmacology
Anions / pharmacology
B-Lymphocytes / metabolism*,  ultrastructure,  virology*
Cell Line, Transformed
Chloride Channels / antagonists & inhibitors,  metabolism*
Cystic Fibrosis Transmembrane Conductance Regulator / metabolism
Herpesvirus 4, Human / physiology*
Ion Channel Gating
Membrane Potentials
Microscopy, Electron
Nickel / pharmacology
Patch-Clamp Techniques
Sodium Chloride / pharmacology
Zinc / pharmacology
Reg. No./Substance:
0/Anions; 0/CFTR protein, human; 0/Chloride Channels; 126880-72-6/Cystic Fibrosis Transmembrane Conductance Regulator; 27816-59-7/4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 7440-02-0/Nickel; 7440-66-6/Zinc; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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