Document Detail


Characterization of redox polymer based electrode and electrochemical behavior for DNA detection.
MedLine Citation:
PMID:  19446067     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Characterization of redox polymer, poly(vinylferrocenium) perchlorate (PVF+ClO4-) coated as a film on Pt electrodes, and the detection of DNA based on the electrochemical behavior of the polymer were described in this study. PVF+ClO4- modified electrodes were prepared by the electrooxidation of poly(vinylferrocene) (PVF), and DNA immobilized polymer modified electrode was then prepared. The characterization of the polymer modified electrodes were performed by scanning tunneling microscopy (STM), Raman spectroscopy and alternating current (AC) impedance. The effects of DNA concentration, pH, and polymer films in various thicknesses based on the electrode response were also investigated. The electrochemical behavior of DNA modified polymer electrode by using double-stranded DNA (dsDNA) or single-stranded DNA (ssDNA) was compared. DNA hybridization was also investigated.
Authors:
Filiz Kuralay; Arzum Erdem; Serdar Abaci; Haluk Ozyörük; Attila Yildiz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-08
Journal Detail:
Title:  Analytica chimica acta     Volume:  643     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-18     Completed Date:  2009-07-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  83-9     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Faculty of Science, Hacettepe University, 06532 Beytepe-Ankara, Turkey.
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MeSH Terms
Descriptor/Qualifier:
DNA / analysis*
Electrochemistry
Electrodes
Ferrous Compounds / chemistry*
Hydrogen-Ion Concentration
Membranes, Artificial
Microscopy, Scanning Tunneling
Oxidation-Reduction
Platinum / chemistry*
Polyvinyls / chemistry*
Spectrum Analysis, Raman
Chemical
Reg. No./Substance:
0/Ferrous Compounds; 0/Membranes, Artificial; 0/Polyvinyls; 0/polyvinylferrocenium; 7440-06-4/Platinum; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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