Document Detail

Characterization of a recombinant thermostable dehalogenase isolated from the hot spring thermophile Sulfolobus tokodaii.
MedLine Citation:
PMID:  19266316     Owner:  NLM     Status:  MEDLINE    
A putative dehalogenase, L-HAD(ST), from the thermophile Sulfolobus tokodaii, was cloned and expressed in Escherichia coli. The recombinant enzyme catalyzes the stereospecific dehalogenation of L-2-haloacids with similar levels of activity as its homolog from mesophiles. L-HAD(ST) remains fully active after being incubated for 4 h at 70 degrees C and tolerates extreme pH conditions ranging from 4 to 10. Furthermore, it can be purified conveniently without the usage of any chromatography method. The high expression yield and easy purification procedure make the recombinant dehalogenase an excellent candidate for biotechnological applications.
Philip G Bachas-Daunert; Stacy A Law; Yinan Wei
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-03-06
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  159     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-05     Completed Date:  2009-12-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  382-93     Citation Subset:  IM    
Paul Laurence Dunbar High School, Lexington, KY 40513, USA.
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MeSH Terms
Cloning, Molecular / methods*
Enzyme Activation
Enzyme Stability
Escherichia coli / physiology
Hot Springs / microbiology*
Hydrolases / chemistry*,  genetics,  metabolism*
Protein Engineering / methods*
Recombinant Proteins / chemistry,  isolation & purification,  metabolism
Sulfolobus / enzymology*,  genetics,  metabolism*
Grant Support
Reg. No./Substance:
0/Recombinant Proteins; EC 3.-/Hydrolases; EC dehalogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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