| Characterization of the recombinant diaminobutyric acid acetyltransferase from Methylophaga thalassica and Methylophaga alcalica. | |
| | |
MedLine Citation:
|
PMID: 18410346 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Diaminobutyric acid acetyltransferase (EctA) catalyzes the acetylation of diaminobutyric acid to gamma-N-acetyl-alpha,gamma-diaminobutyrate with acetyl coenzyme A. This is the second reaction in the ectoine biosynthetic pathway. The recombinant EctA proteins were purified from two moderately halophilic methylotrophic bacteria: Methylophaga thalassica ATCC 33146T and Methylophaga alcalica ATCC 35842T. EctA found in both methylotrophs is a homodimer with a subunit molecular mass of c. 20 kDa and had similar properties with respect to the optimum temperature for activity (30 degrees C), Km for diaminobutyrate (370 or 375 microM) and the absence of requirements for divalent metal ions. The enzyme from M. thalassica exhibited a lower pH optimum and was inhibited both by sodium carbonates and by high ionic strength but to a lesser extent by copper ions. |
| | |
Authors:
|
Ildar I Mustakhimov; Olga N Rozova; Alexander S Reshetnikov; Valentina N Khmelenina; J Colin Murrell; Yuri A Trotsenko |
Related Documents
:
|
3796796 - The nullification by diazepam of haloperidol-induced increases in the level of striatal... 3925996 - A gamma-aminobutyric acid-specific transport mechanism in mammalian kidney. 16463826 - Extracellular amino acid changes in patients during reversible cerebral ischaemia. 18270816 - Post-translational regulation of l-glutamic acid decarboxylase in the brain. 3706716 - Negative ion chemical ionization mass spectrometry of 2,4-dinitrophenyl amino acid esters. 21887646 - Identification of enriched conjugated linoleic acid isomers in cultures of ruminal micr... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-04-09 |
Journal Detail:
|
Title: FEMS microbiology letters Volume: 283 ISSN: 0378-1097 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 2008 Jun |
Date Detail:
|
Created Date: 2008-04-30 Completed Date: 2008-06-23 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: England |
Other Details:
|
Languages: eng Pagination: 91-6 Citation Subset: IM |
Affiliation:
|
Pushchino State University, Pushchino, Moscow region, Russia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetyltransferases
/
biosynthesis* Carbonates / metabolism Cations, Divalent Cloning, Molecular Copper / metabolism Enzyme Stability Hydrogen-Ion Concentration Molecular Weight Osmolar Concentration Piscirickettsiaceae / enzymology* Potassium Chloride / metabolism Recombinant Proteins / biosynthesis Sodium Chloride / metabolism Substrate Specificity Temperature |
| Chemical | |
Reg. No./Substance:
|
0/Carbonates; 0/Cations, Divalent; 0/Recombinant Proteins; 497-19-8/sodium carbonate; 7440-50-8/Copper; 7447-40-7/Potassium Chloride; 7647-14-5/Sodium Chloride; EC 2.3.1.-/2,4-diaminobutyric acid acetyltransferase; EC 2.3.1.-/Acetyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Characterization of Sro1, a novel stress responsive protein in Schizosaccharomyces pombe.
Next Document: Phosphate and sulfur limitation responses in the chloroplast of Chlamydomonas reinhardtii.