Document Detail

Characterization of a recombinant canine coronavirus with a distinct receptor-binding (S1) domain.
MedLine Citation:
PMID:  22609354     Owner:  NLM     Status:  MEDLINE    
Canine alphacoronaviruses (CCoV) exist in two serotypes, type I and II, both of which can cause severe gastroenteritis. Here, we characterize a canine alphacoronavirus, designated CCoV-A76, first isolated in 1976. Serological studies show that CCoV-A76 is distinct from other CCoVs, such as the prototype CCoV-1-71. Efficient replication of CCoV-A76 is restricted to canine cell lines, in contrast to the prototypical type II strain CCoV-1-71 that more efficiently replicates in feline cells. CCoV-A76 can use canine aminopeptidase N (cAPN) receptor for infection of cells, but was unable to use feline APN (fAPN). In contrast, CCoV-1-71 can utilize both. Genomic analysis shows that CCoV-A76 possesses a distinct spike, which is the result of a recombination between type I and type II CCoV, that occurred between the N- and C-terminal domains (NTD and C-domain) of the S1 subunit. These data suggest that CCoV-A76 represents a recombinant coronavirus form, with distinct host cell tropism.
Andrew D Regan; Jean K Millet; Long Ping V Tse; Zach Chillag; Vera D Rinaldi; Beth N Licitra; Edward J Dubovi; Christopher D Town; Gary R Whittaker
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-05-18
Journal Detail:
Title:  Virology     Volume:  430     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-06-18     Completed Date:  2012-08-20     Revised Date:  2014-04-24    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  90-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
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MeSH Terms
Antigens, CD13 / metabolism
Cell Line
Coronavirus, Canine / genetics*,  physiology*
Genome, Viral
Host Specificity / genetics*
Membrane Glycoproteins / chemistry,  genetics*,  metabolism
Molecular Sequence Data
Protein Interaction Domains and Motifs
Protein Subunits / chemistry,  genetics,  metabolism
Receptors, Virus / chemistry,  metabolism*
Recombination, Genetic
Sequence Analysis, RNA
Spike Glycoprotein, Coronavirus
Viral Envelope Proteins / chemistry,  genetics*,  metabolism
Virus Replication
Grant Support
HHSN272200900007C/AI/NIAID NIH HHS; HHSN272200900007C//PHS HHS; T32 AI007618/AI/NIAID NIH HHS; T32AI007618/AI/NIAID NIH HHS; T35 AI007227/AI/NIAID NIH HHS
Reg. No./Substance:
0/Membrane Glycoproteins; 0/Protein Subunits; 0/Receptors, Virus; 0/Spike Glycoprotein, Coronavirus; 0/Viral Envelope Proteins; EC, CD13

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