| Characterization of a recombinant canine coronavirus with a distinct receptor-binding (S1) domain. | |
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MedLine Citation:
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PMID: 22609354 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Canine alphacoronaviruses (CCoV) exist in two serotypes, type I and II, both of which can cause severe gastroenteritis. Here, we characterize a canine alphacoronavirus, designated CCoV-A76, first isolated in 1976. Serological studies show that CCoV-A76 is distinct from other CCoVs, such as the prototype CCoV-1-71. Efficient replication of CCoV-A76 is restricted to canine cell lines, in contrast to the prototypical type II strain CCoV-1-71 that more efficiently replicates in feline cells. CCoV-A76 can use canine aminopeptidase N (cAPN) receptor for infection of cells, but was unable to use feline APN (fAPN). In contrast, CCoV-1-71 can utilize both. Genomic analysis shows that CCoV-A76 possesses a distinct spike, which is the result of a recombination between type I and type II CCoV, that occurred between the N- and C-terminal domains (NTD and C-domain) of the S1 subunit. These data suggest that CCoV-A76 represents a recombinant coronavirus form, with distinct host cell tropism. |
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Authors:
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Andrew D Regan; Jean K Millet; Long Ping V Tse; Zach Chillag; Vera D Rinaldi; Beth N Licitra; Edward J Dubovi; Christopher D Town; Gary R Whittaker |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-05-18 |
Journal Detail:
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Title: Virology Volume: 430 ISSN: 1096-0341 ISO Abbreviation: Virology Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-06-18 Completed Date: 2012-08-20 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 90-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Microbiology and Immunology, Veterinary Medical Center, Cornell University, Ithaca, NY 14853, United States. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AEQ61967 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD13 / metabolism Cats Cell Line Coronavirus, Canine / genetics*, physiology* Dogs Genome, Viral Host Specificity / genetics* Membrane Glycoproteins / chemistry, genetics*, metabolism Molecular Sequence Data Phylogeny Protein Interaction Domains and Motifs Protein Subunits / chemistry, genetics, metabolism Receptors, Virus / chemistry, metabolism* Recombination, Genetic Sequence Analysis, RNA Viral Envelope Proteins / chemistry, genetics*, metabolism Virus Replication |
| Grant Support | |
ID/Acronym/Agency:
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HHSN272200900007C//PHS HHS; T32 AI007618/AI/NIAID NIH HHS; T32AI007618/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Membrane Glycoproteins; 0/Protein Subunits; 0/Receptors, Virus; 0/Viral Envelope Proteins; 107476-75-5/spike glycoprotein, coronavirus; EC 3.4.11.2/Antigens, CD13 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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