| Characterization of a recombinant L-fucose isomerase from Caldicellulosiruptor saccharolyticus that isomerizes L-fucose, D-arabinose, D-altrose, and L-galactose. | |
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MedLine Citation:
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PMID: 19856146 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A recombinant L-fucose isomerase from Caldicellulosiruptor saccharolyticus was purified as a single 68 kDa band with an activity of 76 U mg(-1). The molecular mass of the native enzyme was 204 kDa as a trimer. The maximum activity for L-fucose isomerization was at pH 7 and 75 degrees C in the presence of 1 mM Mn(2+). Its half-life at 70 degrees C was 6.1 h. For aldose substrates, the enzyme displayed activity in decreasing order for L-fucose, with a k (cat) of 11,910 min(-1) and a K (m) of 140 mM, D-arabinose, D-altrose, and L-galactose. These aldoses were converted to the ketoses L-fuculose, D-ribulose, D-psicose, and L-tagatose, respectively, with 24, 24, 85, 55% conversion yields after 3 h. |
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Authors:
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Yo-Han Ju; Deok-Kun Oh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-25 |
Journal Detail:
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Title: Biotechnology letters Volume: 32 ISSN: 1573-6776 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-21 Completed Date: 2010-03-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 299-304 Citation Subset: IM |
Affiliation:
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Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, South Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aldose-Ketose Isomerases
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chemistry*,
genetics,
metabolism* Arabinose / chemistry* Enzyme Activation Fucose / chemistry* Galactose / chemistry* Isomerism Recombinant Proteins / chemistry*, metabolism Thermoanaerobacterium / enzymology*, genetics |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Proteins; 147-81-9/Arabinose; 26566-61-0/Galactose; 3713-31-3/Fucose; EC 5.3.1/Aldose-Ketose Isomerases; EC 5.3.1.3/D-arabinose isomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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