Document Detail


Characterization of the reaction products of deoxyguanosine with the anticancer agent BFNU and BFNU-1,1,1',1'-d4 in different buffers by high-performance liquid chromatography/atmospheric pressure ionization tandem mass spectrometry.
MedLine Citation:
PMID:  2400854     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The products of the reaction of the anticancer agent 1,3-bis(2-fluoroethyl)-1-nitrosourea (BFNU) and BFNU-1,1,1',1'-d4 with the DNA base deoxyguanosine were characterized by applying high-performance liquid chromatography (HPLC)/tandem mass spectrometry. The total effluent from the HPLC column was introduced into the atmospheric pressure ionization (API) source of a triple-quadrupole mass spectrometer via a heated nebulizer. The gasified mixture produced from the heated nebulizer was exposed to corona discharge ionization which led to generation of gas-phase chemical ionization type of ions. The LC/API mass spectrometry produced ions and the tandem mass spectra allowed unambiguous identification, and assignment of positions of the deuterium atoms, in the products of the reaction under a variety of experimental conditions. The identification and characterization of a variety of 7-(2'-haloethyl)guanine derivatives among the reaction products provide confirmation of a proposed mechanism for the action of BFNU on DNA bases.
Authors:
M G Ikonomou; A Naghipur; J W Lown; P Kebarle
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biomedical & environmental mass spectrometry     Volume:  19     ISSN:  0887-6134     ISO Abbreviation:  Biomed. Environ. Mass Spectrom.     Publication Date:  1990 Jul 
Date Detail:
Created Date:  1990-10-26     Completed Date:  1990-10-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8603224     Medline TA:  Biomed Environ Mass Spectrom     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  434-46     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of Alberta, Edmonton, Canada.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents*
Atmospheric Pressure
Bromides
Buffers*
Chemical Phenomena
Chemistry
Chromatography, High Pressure Liquid
Deoxyguanosine*
Guanine / analogs & derivatives
Hydrolysis
Mass Spectrometry
Nitrosourea Compounds*
Potassium
Potassium Compounds*
Tromethamine
Grant Support
ID/Acronym/Agency:
CA/GM 2 RO1 21488-12/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Bromides; 0/Buffers; 0/Nitrosourea Compounds; 0/Potassium Compounds; 125973-91-3/7-(2'-fluoroethyl)guanine; 13908-91-3/bis-(fluoroethyl)nitrosourea; 53498-52-5/N(7)-hydroxyethylguanine; 73-40-5/Guanine; 7440-09-7/Potassium; 77-86-1/Tromethamine; 7758-02-3/potassium bromide; 961-07-9/Deoxyguanosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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