Document Detail

Characterization of rat placental lactogen-alpha (PL-alpha) with an antipeptide antibody directed against rat PLs.
MedLine Citation:
PMID:  7951543     Owner:  NLM     Status:  MEDLINE    
We have already reported that the serum of the mid-pregnant rat contains placental lactogen-alpha (PL-alpha), which has a higher molecular weight than PL-I when analysed by gel-filtration chromatography. In order to characterize PL-alpha, we prepared antipeptide antisera directed against the hydrophilic regions of authentic PLs and developed RIAs with them. With these RIAs, antiserum AI-86 reacted only with PL-I, whereas antiserum AII-86 reacted with both PL-alpha and PL-I, which indicates that the antibody recognition sites of these molecules are not identical and their core proteins differ distinctly. As the use of the RIAs with AI-86 and AII-86 in combination enabled PL-alpha and PL-I to be discriminated, the glycoresidues and hydrophobicity of PL-alpha were analyzed further. Analysis with hydrophobic chromatography revealed that PL-alpha was less hydrophobic than PL-I and, furthermore, PL-alpha, but not PL-I, bound specifically to a wheat germ agglutinin (WGA) lectin column. Therefore, in view of their different amino acid sequences and glycoresidues, PL-alpha and PL-I are distinct molecular entities.
N Hattori; M Wakimasu; M Takahashi; M Hirosawa; K Shiota; T Ogawa
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrine journal     Volume:  40     ISSN:  0918-8959     ISO Abbreviation:  Endocr. J.     Publication Date:  1993 Dec 
Date Detail:
Created Date:  1994-12-08     Completed Date:  1994-12-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9313485     Medline TA:  Endocr J     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  727-35     Citation Subset:  IM    
Laboratory of Cellular Biochemistry, University of Tokyo, Japan.
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MeSH Terms
Amino Acid Sequence
Antibodies / immunology*
Antibody Specificity
Chemistry, Physical
Chromatography, Affinity
Chromatography, Gel
Chromatography, High Pressure Liquid
Enzyme-Linked Immunosorbent Assay
Immune Sera
Molecular Sequence Data
Molecular Weight
Peptide Fragments / chemical synthesis,  immunology*
Physicochemical Phenomena
Placental Lactogen / chemistry
Rats, Wistar
Sequence Alignment
Sequence Homology, Amino Acid
Wheat Germ Agglutinins
Reg. No./Substance:
0/Antibodies; 0/Immune Sera; 0/Peptide Fragments; 0/Wheat Germ Agglutinins; 0/placental lactogen II; 149146-52-1/placental lactogen I, rat; 9035-54-5/Placental Lactogen

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