Document Detail

Characterization of a rat oral squamous cell carcinoma cell line UHG-RaC '93 induced by 4-nitroquinoline-1-oxide in vivo.
MedLine Citation:
PMID:  7586205     Owner:  NLM     Status:  MEDLINE    
This study describes several characteristics of a cell line, UHG-RaC '93 derived from rat oral squamous cell carcinoma induced by the carcinogen 4-nitroquinoline-1-oxide (4NQO). The cell line was established from explant cultures without support of fibroblast feeder cells and continued for > 30 passages. UHG-RaC '93 had a high mitotic rate with a population doubling time of 25 h and a high rate of squame production. The first passage had a low colony-forming efficiency in agarose gel, whereas later passages did not grow at all in semi-solid medium. Phenotype selection was furthermore apparent from a gradual increase of the trypsin-detachment time. Cytogenetic analysis showed that UHG-RaC '93 was hypotetraploid with an average of 74 chromosomes. Abnormalities compared to the normal karyotype were assessed and consisted mainly of breakpoints at (1)(q5?3), (3)(p1), (3)(q11q23), (11)(p?11), (13)(p13) and a derivative (12)t(12;13)(q10;q10). The karyotype remained stable for at least 26 passages. The expression of typical epithelioid markers like cytokeratins and desmoglein corresponded with normal rat oral keratinocytes. However expression of alpha 6 beta 4-integrin was altered. Squame production, immunophenotype and anchorage dependency indicated that UHG-RaC '93 had the same features of a well-differentiated carcinoma with a low degree of agressiveness as the original tumour. The stable karyotype of this cell line provides a basis for further analysis of the effect of 4NQO on the genotype, phenotype and behaviour of rat oral keratinocytes.
M Witjes; J Scholma; E van Drunen; J L Roodenburg; G Mesander; A Hagemeijer; A M Tomson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Carcinogenesis     Volume:  16     ISSN:  0143-3334     ISO Abbreviation:  Carcinogenesis     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1995-12-28     Completed Date:  1995-12-28     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2825-32     Citation Subset:  IM    
Department of Oral and Maxillofacial Surgery, University Hospital Groningen, The Netherlands.
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MeSH Terms
4-Nitroquinoline-1-oxide / toxicity*
Carcinogens / toxicity*
Carcinoma, Squamous Cell / chemically induced,  genetics,  pathology*
Cells, Cultured
Chromosome Aberrations
Fluorescent Antibody Technique
Integrins / analysis
Mouth Neoplasms / chemically induced,  genetics,  pathology*
Neoplasm Proteins / analysis
Rats, Wistar
Tumor Cells, Cultured
Reg. No./Substance:
0/Carcinogens; 0/Integrins; 0/Neoplasm Proteins; 56-57-5/4-Nitroquinoline-1-oxide

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