Document Detail


Characterization of a rat hypoxia-ischemia model where duration of hypoxia is determined by seizure activity.
MedLine Citation:
PMID:  21320527     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Perinatal and early childhood asphyxia is common, debilitating and has few efficacious treatments. A hypoxia ischemia (HI) rat model that involves a unilateral ligation of the common carotid artery followed by a 60minute period of 8% oxygen hypoxia is often used to test proposed treatments. However, this HI protocol produces inconsistent infarction volumes due to the variability of individual rats to compensate for the ligated artery and hypoxia. Therefore, this HI model is problematic for experiments that prevent measurement of infarction volume, such as those that require analysis of homogenised brain tissue. We therefore aimed to find a simple and non-invasive predictor of infarction volume. Observations made prior, during and following HI in p26 rats showed that weight change 24hours following surgery was a strong predictor of infarction volume. The occurrence of a tonic clonic seizure during hypoxia was highly correlated with success of inducing an infarction, and for this reason we assessed whether ceasing the hypoxia for each rat following a tonic clonic seizure would produce a more consistent infarction volume. Using this procedure, infarction volumes measured at 3 and 15days after surgery were significantly less variable, resulting in considerable improvements in statistical power compared with the original model.
Authors:
Jack R Rivers; Brad Sutherland; John C Ashton
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-11
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  -     ISSN:  1872-678X     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-2-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier B.V.
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