Document Detail


Characterization of purified rabbit uterine renin: influence of pregnancy on uterine inactive renin.
MedLine Citation:
PMID:  3536455     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using specific antibody raised against renal renin, we have documented that the majority of the uterine renin-like activity in gravid and nongravid uteri is immunoreactive renin. To characterize its physiochemical properties, we obtained highly purified uterine renin by two affinity chromatographic steps, pepstatin and antirenin. Uterine renin has a pH optimum of 6, an apparent mol wt of 38K, and a Km of 1.7 microM for homologous substrate. These properties are identical to those of renal renin and are not influenced by the pregnant state. In the basal state, an inactive form of the uterine enzyme constitute 55 +/- 10% of the total uterine renin. During pregnancy, active renin increased 40-fold as inactive renin fell to 4 +/- 3% of the total renin concentration. The renal renin concentration fell as plasma renin increased during pregnancy. These data suggest that the increased uterine renin concentrations during pregnancy are probably due to increased local production and conversion of renin precursor to the active enzyme. This stimulation of the uterine renin level appears to be independent of renal renin.
Authors:
V J Dzau; D Gonzalez; K Ellison; S Churchill; N Emmett
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  120     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-01-20     Completed Date:  1987-01-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  358-64     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Enzyme Activation
Female
Hydrogen-Ion Concentration
Kidney / enzymology
Kinetics
Molecular Weight
Pregnancy
Pregnancy, Animal / metabolism*
Rabbits
Renin / isolation & purification,  metabolism*
Uterus / enzymology*
Grant Support
ID/Acronym/Agency:
HD-05755/HD/NICHD NIH HHS; HL-05544/HL/NHLBI NIH HHS; HL-35610/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
EC 3.4.23.15/Renin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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