Document Detail


Characterization of proteolytic activities in embryos of Xenopus laevis.
MedLine Citation:
PMID:  3066576     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. Proteolytic activities in early embryos of Xenopus laevis exhibited maximum levels at pH 3.2, 5.6 and 7.2 when 3H-BSA was used as substrate, and the maximum proteolytic activity at pH 3.2 was several thousand-fold higher during the tail bud stage than in the unfertilized egg. 2. The proteolytic activity at pH 3.2 was separated into two fractions by gel chromatography. One fraction corresponded to a mol. wt of about 40,000 and its activity was inhibited by thiol protease inhibitors. The other appeared to be a protease of much higher mol. wt. 3. The maximum activities at pH 5.6 and 7.2 appear to correspond to proteins of mol. wt greater than 1,000,000.
Authors:
S Miyata; H K Kihara
Related Documents :
2197026 - Proteolytic measurement of mitochondrial aspartate aminotransferase in human serum.
6309756 - Structural study on the active site of porcine pepsin and rhizopus chinensis acid prote...
8305526 - Biotinylated isocoumarins, new inhibitors and reagents for detection, localization, and...
1629666 - Presence of a trypsin-like protease in starfish sperm acrosome.
10194396 - Hydrazide-functionalized poly(2-hydroxyethyl methacrylate) microspheres for immobilizat...
10929006 - Purification and some properties of udp-xylosyltransferase of rat ear cartilage.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Comparative biochemistry and physiology. B, Comparative biochemistry     Volume:  91     ISSN:  0305-0491     ISO Abbreviation:  Comp. Biochem. Physiol., B     Publication Date:  1988  
Date Detail:
Created Date:  1989-03-30     Completed Date:  1989-03-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2984730R     Medline TA:  Comp Biochem Physiol B     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  651-6     Citation Subset:  IM    
Affiliation:
Laboratory of Research for Biosynthesis and Metabolism, Keio University, School of Medicine, Tokyo, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cysteine Endopeptidases / isolation & purification,  metabolism
Dactinomycin / pharmacology
Dithiothreitol / pharmacology
Embryo, Nonmammalian / enzymology
Hydrogen-Ion Concentration
Molecular Weight
Peptide Hydrolases / isolation & purification*,  metabolism
Xenopus laevis / metabolism*
Chemical
Reg. No./Substance:
3483-12-3/Dithiothreitol; 50-76-0/Dactinomycin; EC 3.4.-/Peptide Hydrolases; EC 3.4.22.-/Cysteine Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Comparison of serum protein inhibitors from various mammals, chicken and silkworms against four prot...
Next Document:  Genomic DNA sequence polymorphism of Trypanosoma (Schizotrypanum) cruzi isolates with phenotypic var...